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Hokkaido University Collection of Scholarly and Academic Papers >
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Complete Sructure of Nuclear rDNA of the Obligate Plant Parasite Plasmodiophora brassicae : Intraspecific Polymorphisms in the Exon and Group I Intron of the Large Subunit rDNA
| Title: | Complete Sructure of Nuclear rDNA of the Obligate Plant Parasite Plasmodiophora brassicae : Intraspecific Polymorphisms in the Exon and Group I Intron of the Large Subunit rDNA |
| Authors: | Niwa, Rieko Browse this author | | Kawahara, Ai Browse this author | | Murakami, Hiroharu Browse this author | | Tanaka, Shuhei Browse this author | | Ezawa, Tatsuhiro Browse this author |
| Keywords: | Cercozoa | | divergent domain in LSU rDNA | | group I intron | | intraspecific polymorphism | | nuclear rDNA | | Plasmodiophora brassicae |
| Issue Date: | Jul-2011 |
| Publisher: | Elsevier |
| Journal Title: | Protist |
| Volume: | 162 |
| Issue: | 3 |
| Start Page: | 423 |
| End Page: | 434 |
| Publisher DOI: | 10.1016/j.protis.2011.02.005 |
| PMID: | 21497131 |
| Abstract: | Plasmodiophora brassicae is a soil-borne obligate intracellular parasite in the phylum Cercozoa of the Rhizaria that causes clubroot disease of crucifer crops. To control the disease, understanding the distribution and infection routes of the pathogen is essential, and thus development of reliable molecular markers to discriminate geographic populations is required. In this study, the nuclear ribosomal RNA gene (rDNA) repeat unit of P. brassicae was determined, with particular emphasis on the structure of large subunit (LSU) rDNA, in which polymorphic regions were expected to be present. The complete rDNA complex was 9513 bp long, which included the small subunit, 5.8S and LSU rDNAs as well as the internal transcribed spacer and intergenic spacer regions. Among eight field populations collected from throughout Honshu Island, Japan, a 1.1 kbp region of the LSU rDNA, including the divergent 8 domain, exhibited intraspecific polymorphisms that reflected geographic isolation of the populations. Two new group I introns were found in this region in six out of the eight populations, and the sequences also reflected their geographic isolation. The polymorphic region found in this study may have potential for the development of molecular markers for discrimination of field populations/isolates of this organism. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/46765 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 江澤 辰広
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