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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
An intact mitochondrial cox1 gene and a pseudogene with different genomic configurations are present in apple cultivars 'Golden Delicious' and 'Delicious': Evolutionary aspects
| Title: | An intact mitochondrial cox1 gene and a pseudogene with different genomic configurations are present in apple cultivars 'Golden Delicious' and 'Delicious': Evolutionary aspects |
| Authors: | Wakatsuki, Akihito Browse this author | | Kitazaki, Kazuyoshi Browse this author | | Kato, Sumie Browse this author | | Kishima, Yuji Browse this author →KAKEN DB | | Mikami, Tetsuo Browse this author |
| Issue Date: | 26-Aug-2011 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Scientia Horticulturae |
| Volume: | 130 |
| Issue: | 1 |
| Start Page: | 49 |
| End Page: | 53 |
| Publisher DOI: | 10.1016/j.scienta.2011.05.037 |
| Abstract: | We have characterized the mitochondrial cox1 gene copies in two apple cultivars 'Golden Delicious' and 'Delicious'. Both the cultivars contained an intact copy and a truncated copy of cox1. The intact 'Golden Delicious' and 'Delicious' cox1 genes, designated G-cox1 and D-cox1, respectively, were both found to be actually transcribed to give an RNA of approximately 1.7 kb. The two intact cox1 and two truncated copies (G-φcox1 and D-φcox1) shared a common 1115-bp segment flanked by four combinations of two different 5'- and 3'-sequences. PCR assay demonstrated that the configurations bearing G-cox1 and G-φcox1 existed in substoichiometric amounts within the mitochondrial genome of 'Delicious' whereas substoichiometric molecules carrying D-φcox1 were present in the 'Golden Delicious' mitochondrial genome. Although ancestor/descendant relationships cannot be inferred between the G-cox1 and D-cox1 arrangements, the results led us to hypothesize that (1) the 1115-bp segment containing part of the progenitor cox1 was duplicated, thereby generating a pseudo-cox1 copy, and (2) this was followed by homologous recombination across a portion of the 1115-bp repeats which gave rise to the descendant cox1 and pseudo-cox1 arrangements. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/48642 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 貴島 祐治
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