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Hokkaido University Collection of Scholarly and Academic Papers >
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Characterization of phase-II conjugation reaction of polycyclic aromatic hydrocarbons in fish species : Unique pyrene metabolism and species specificity observed in fish species
| Title: | Characterization of phase-II conjugation reaction of polycyclic aromatic hydrocarbons in fish species : Unique pyrene metabolism and species specificity observed in fish species |
| Authors: | Ikenaka, Yoshinori Browse this author →KAKEN DB | | Oguri, Mami Browse this author | | Saengtienchai, Aksorn Browse this author | | Nakayama, Shouta M. M. Browse this author →KAKEN DB | | Ijiri, Shigeho Browse this author →KAKEN DB | | Ishizuka, Mayumi Browse this author →KAKEN DB |
| Keywords: | Fish | | Pyrene | | Conjugation | | Polycyclic aromatic hydrocarbons (PAHs) | | Metabolism | | Inter-species difference |
| Issue Date: | Sep-2013 |
| Publisher: | Elsevier |
| Journal Title: | Environmental Toxicology and Pharmacology |
| Volume: | 36 |
| Issue: | 2 |
| Start Page: | 567 |
| End Page: | 578 |
| Publisher DOI: | 10.1016/j.etap.2013.05.018 |
| PMID: | 23834960 |
| Abstract: | Metabolic activity, particularly conjugation, was examined in fish by analyzing pyrene (a four-ring, polycyclic aromatic hydrocarbon) metabolites using high-performance liquid chromatography (HPLC) with fluorescence detector (FD), a mass spectrometry (MS) system, and kinetic analysis of conjugation enzymes. Fourteen fresh water fish species, including Danio rerio and Orizias latipes, were exposed to aqueous pyrene, and the resulting metabolites were collected. Identification of pyrene metabolites by HPLC/FD and ion-trap MS indicated that the major metabolites were pyrene glucuronide and pyrene sulfate in all 14 species. Differences were observed in pyrene glucuronide:pyrene sulfate ratio and in the total amount of pyrene conjugates excreted between fish species. Furthermore, a correlation was found between the amount of pyrene glucuronide present and the total amount of the pyrene metabolite eliminated. Kinetic analysis of conjugation by hepatic microsomes in vitro indicated that the differences in excreted metabolites reflected the differences in enzymatic activities. (C) 2013 Elsevier B.V. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/53434 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 池中 良徳
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