| Title: | Decreased secretion of Paneth cell α-defensins in graft-versus-host disease |
| Other Titles: | Fecal α-defensins in GVHD |
| Authors: | Eriguchi, Y. Browse this author |
| Nakamura, K. Browse this author |
| Hashimoto, D. Browse this author |
| Shimoda, S. Browse this author |
| Shimono, N. Browse this author |
| Akashi, K. Browse this author |
| Ayabe, T. Browse this author |
| Teshima, T.8 Browse this author →KAKEN DB |
| Authors(alt): | Teshima, Takanori8 |
| Keywords: | graft-versus-host disease |
| hematopoietic stem cell transplantation |
| α-defensins |
| cryptdin |
| mouse model |
| Paneth cells |
| fecal testing |
| microbiota |
| Issue Date: | Oct-2015 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Transplant infectious disease |
| Volume: | 17 |
| Issue: | 5 |
| Start Page: | 702 |
| End Page: | 706 |
| Publisher DOI: | 10.1111/tid.12423 |
| PMID: | 26198302 |
| Abstract: | Background: Intestinal microbial ecology is actively regulated by Paneth cell-derived antimicrobial peptides, α-defensins. Graft-versus-host disease (GVHD) is a major complication of allogeneic hematopoietic stem cell transplantation (SCT). We previously demonstrated that Paneth cells are targeted by GVHD, and their expression of antimicrobial peptide α-defensins is impaired, leading to a loss of physiological diversity among the microflora and development of bloodstream infection. Herein, we evaluated whether fecal levels of α-defensins could be surrogate marker of intestinal dysbiosis. Methods: We directly measured α-defensin cryptdin-1 (Crp1) in fecal pellets of mice with GVHD by using a novel enzyme-linked immunosorbent assay. Results: Fecal levels of Crp1 were significantly decreased in mice with GVHD but unchanged in mice without GVHD after SCT. These were correlated with intestinal flora diversity. Conclusion: We demonstrate a link between reduced secretion of Paneth cell α-defensins and dysbiosis of intestinal flora in GVHD. Fecal levels of α-defensins could be surrogate markers for intestinal microbial homeostasis. |
| Rights: | This is the peer reviewed version of the following article: Y. Eriguchi, K. Nakamura, D. Hashimoto, S. Shimoda, N. Shimono, K. Akashi, T. Ayabe, T. Teshima. Decreased secretion of Paneth cell α-defensins in graft-versus-host disease. Transpl Infect Dis 2015: 17: 702–706., which has been published in final form at http://dx.doi.org/10.1111/tid.12423. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/62922 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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