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Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase
Title: | Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase |
Authors: | Yokoo, Toshinori Browse this author | Matsumoto, Ken'ichiro Browse this author →KAKEN DB | Ooba, Takashi Browse this author | Morimoto, Kenjiro Browse this author | Taguchi, Seiichi Browse this author →KAKEN DB |
Keywords: | polyhydroxyalkanoate | polyhydroxybutyrate | biodegradable plastic |
Issue Date: | Jun-2015 |
Journal Title: | Bioscience, Biotechnology, and Biochemistry |
Volume: | 79 |
Issue: | 6 |
Start Page: | 986 |
End Page: | 988 |
Publisher DOI: | 10.1080/09168451.2014.1002448 |
PMID: | 25647430 |
Abstract: | Highly active mutant of NADPH-dependent acetoacetyl-CoA reductase (PhaB) was expressed in Nicotiana tabacum cv. Bright Yellow-2 cultured cells to produce poly(3-hydroxybutyrate) [P(3HB)]. The mutated PhaB increased P(3HB) content by three-fold over the control, indicating that the mutant was a versatile tool for P(3HB) production. Additionally, the PhaB-catalyzed reaction was suggested to be a rate-limiting step of P(3HB) biosynthesis in tobacco BY-2 cells. |
Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on June 2015, available online: http://wwww.tandfonline.com/10.1080/09168451.2014.1002448. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/63178 |
Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松本 謙一郎
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