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Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase

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Title: Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase
Authors: Yokoo, Toshinori Browse this author
Matsumoto, Ken'ichiro Browse this author →KAKEN DB
Ooba, Takashi Browse this author
Morimoto, Kenjiro Browse this author
Taguchi, Seiichi Browse this author →KAKEN DB
Keywords: polyhydroxyalkanoate
polyhydroxybutyrate
biodegradable plastic
Issue Date: Jun-2015
Journal Title: Bioscience, Biotechnology, and Biochemistry
Volume: 79
Issue: 6
Start Page: 986
End Page: 988
Publisher DOI: 10.1080/09168451.2014.1002448
PMID: 25647430
Abstract: Highly active mutant of NADPH-dependent acetoacetyl-CoA reductase (PhaB) was expressed in Nicotiana tabacum cv. Bright Yellow-2 cultured cells to produce poly(3-hydroxybutyrate) [P(3HB)]. The mutated PhaB increased P(3HB) content by three-fold over the control, indicating that the mutant was a versatile tool for P(3HB) production. Additionally, the PhaB-catalyzed reaction was suggested to be a rate-limiting step of P(3HB) biosynthesis in tobacco BY-2 cells.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on June 2015, available online: http://wwww.tandfonline.com/10.1080/09168451.2014.1002448.
Type: article (author version)
URI: http://hdl.handle.net/2115/63178
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松本 謙一郎

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