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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
A Genomic Region Transcribed Into a Long Noncoding RNA Interacts With the Prss42/Tessp-2 Promoter in Spermatocytes During Mouse Spermatogenesis, and Its Flanking Sequences Can Function as Enhancers
| Title: | A Genomic Region Transcribed Into a Long Noncoding RNA Interacts With the Prss42/Tessp-2 Promoter in Spermatocytes During Mouse Spermatogenesis, and Its Flanking Sequences Can Function as Enhancers |
| Authors: | Yoneda, Ryoma Browse this author | | Satoh, Yui Browse this author | | Yoshida, Ikuya Browse this author | | Kawamura, Shohei Browse this author | | Kotani, Tomoya Browse this author | | Kimura, Atsushi P. Browse this author →KAKEN DB |
| Issue Date: | Jun-2016 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Molecular reproduction and development |
| Volume: | 83 |
| Issue: | 6 |
| Start Page: | 541 |
| End Page: | 557 |
| Publisher DOI: | 10.1002/mrd.22650 |
| PMID: | 27111572 |
| Abstract: | Spermatogenesis is regulated by many meiotic stage-specific genes, but how they coordinate the many individual processes is not fully understood. The Prss/Tessp gene cluster is located on mouse chromosome 9F2-F3, and the three genes at this site (Prss42/Tessp-2, Prss43/Tessp-3, and Prss44/Tessp-4) are specifically activated during meiosis in pachytene spermatocytes. We searched for DNase I hypersensitive sites (HSs) and long noncoding RNAs (lncRNAs) at the Prss/Tessp locus to elucidate how they are activated. We found eight DNase I HSs, three of which were testis germ cell-specific at or close to the Prss42/Tessp-2 promoter, and a testisspecific lncRNA, lncRNA-HSVIII, that was transcribed from a region adjacent to the Prss42/Tessp-2 gene. lncRNA-HSVIII transcripts localized to nuclei of most pachytene spermatocytes and the cytosol of stage-X pachytene spermatocytes and spermatids. Chromosome conformation capture revealed that the lncRNA-HSVIII locus specifically interacted with the Prss42/Tessp-2 promoter in primary and secondary spermatocytes. A 5.8-kb genome sequence, encompassing the entire lncRNA-HSVIII sequence and its flanking regions, significantly increased Prss42/Tessp-2 promoter activity using a reporter-gene assay, yet this construct did not change lncRNA-HSVIII expression, indicating that the elevated promoter activity was likely through enhancer activity. Indeed, both upstreamand downstream regions of the lncRNA-HSVIII sequence significantly increased Prss42/Tessp-2 promoter activity. Our data therefore identified the direct interaction of a genomic region in the lncRNA-HSVIII locus with the Prss42/Tessp-2 promoter in spermatocytes, and suggested that sequences adjacent to the lncRNA function as enhancers for the Prss42/Tessp-2 gene. (C) 2016 Wiley Periodicals, Inc. |
| Rights: | This is the peer reviewed version of the following article: Yoneda, R., Satoh, Y., Yoshida, I., Kawamura, S., Kotani, T. and Kimura, A. P. (2016), A genomic region transcribed into a long noncoding RNA interacts with the Prss42/Tessp-2 promoter in spermatocytes during mouse spermatogenesis, and its flanking sequences can function as enhancers. Mol. Reprod. Dev., 83: 541–557, which has been published in final form at doi:10.1002/mrd.22650. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/65841 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 敦
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