Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Information Science and Technology / Faculty of Information Science and Technology >
Peer-reviewed Journal Articles, etc >
High-Speed In Situ Observation System for Sonoporation of Cells With Size- and Position-Controlled Microbubbles
Title: | High-Speed In Situ Observation System for Sonoporation of Cells With Size- and Position-Controlled Microbubbles |
Authors: | Kudo, Nobuki Browse this author →KAKEN DB |
Keywords: | Biological effects and dosimetry | biophysical mechanisms | therapeutics | ultrasound contrast agents |
Issue Date: | Jan-2017 |
Publisher: | IEEE (Institute of Electrical and Electronics Engineers) |
Journal Title: | IEEE transactions on ultrasonics ferroelectrics and frequency control |
Volume: | 64 |
Issue: | 1 |
Start Page: | 273 |
End Page: | 280 |
Publisher DOI: | 10.1109/TUFFC.2016.2606551 |
Abstract: | A high-speed in situ microscopic observation system developed for basic studies on mechanisms of sonoporation is introduced in this paper. The main part of the system is an inverted-type fluorescence microscope, and a high-speed camera of 20 MHz in a maximum framing rate was used to visualize the dynamics of cavitation bubbles that causes a sonoporation effect. Differential interference contrast and fluorescence techniques were used for sensitive visualization of cell changes during sonoporation. The system is also equipped with optical tweezers that can move a microbubble of several microns in size by using a donut-shaped light beam. In situ microscopic observation of sonoporation was carried out using a cell with a size-and position-controlled microbubble. The experimental results showed that the ability of cells to repair sonoporation-induced damage depends on their membrane tension, indicating the usefulness of the observation system as a basic tool for the investigation of sonoporation phenomena. |
Rights: | © 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/66187 |
Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 工藤 信樹
|