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High-Speed In Situ Observation System for Sonoporation of Cells With Size- and Position-Controlled Microbubbles

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/66187

Title: High-Speed In Situ Observation System for Sonoporation of Cells With Size- and Position-Controlled Microbubbles
Authors: Kudo, Nobuki Browse this author →KAKEN DB
Keywords: Biological effects and dosimetry
biophysical mechanisms
therapeutics
ultrasound contrast agents
Issue Date: Jan-2017
Publisher: IEEE (Institute of Electrical and Electronics Engineers)
Journal Title: IEEE transactions on ultrasonics ferroelectrics and frequency control
Volume: 64
Issue: 1
Start Page: 273
End Page: 280
Publisher DOI: 10.1109/TUFFC.2016.2606551
Abstract: A high-speed in situ microscopic observation system developed for basic studies on mechanisms of sonoporation is introduced in this paper. The main part of the system is an inverted-type fluorescence microscope, and a high-speed camera of 20 MHz in a maximum framing rate was used to visualize the dynamics of cavitation bubbles that causes a sonoporation effect. Differential interference contrast and fluorescence techniques were used for sensitive visualization of cell changes during sonoporation. The system is also equipped with optical tweezers that can move a microbubble of several microns in size by using a donut-shaped light beam. In situ microscopic observation of sonoporation was carried out using a cell with a size-and position-controlled microbubble. The experimental results showed that the ability of cells to repair sonoporation-induced damage depends on their membrane tension, indicating the usefulness of the observation system as a basic tool for the investigation of sonoporation phenomena.
Rights: © 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
Type: article (author version)
URI: http://hdl.handle.net/2115/66187
Appears in Collections:情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 工藤 信樹

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