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Hokkaido University Collection of Scholarly and Academic Papers >
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Dietary alpha-cyclodextrin reduces atherosclerosis and modifies gut flora in apolipoprotein E-deficient mice
| Title: | Dietary alpha-cyclodextrin reduces atherosclerosis and modifies gut flora in apolipoprotein E-deficient mice |
| Authors: | Sakurai, Toshihiro Browse this author | | Sakurai, Akiko Browse this author | | Chen, Ye Browse this author | | Vaisman, Boris L. Browse this author | | Amar, Marcelo J. Browse this author | | Pryor, Milton Browse this author | | Thacker, Seth G. Browse this author | | Zhang, Xue Browse this author | | Wang, Xujing Browse this author | | Zhang, Yubo Browse this author | | Zhu, Jun Browse this author | | Yang, Zhi-Hong Browse this author | | Freeman, Lita A. Browse this author | | Remaley, Alan T. Browse this author |
| Keywords: | Atherosclerosis | | Cyclodextrin | | Dietary fiber | | Gut flora |
| Issue Date: | 16-Aug-2017 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Molecular nutrition & food research |
| Volume: | 61 |
| Issue: | 8 |
| Start Page: | 1600804 |
| Publisher DOI: | 10.1002/mnfr.201600804 |
| PMID: | 28102587 |
| Abstract: | Scope: alpha-Cyclodextrin (alpha-CD), a cyclic polymer of glucose, has been shown to lower plasma cholesterol in animals and humans; however, its effect on atherosclerosis has not been previously described. Methods and results: apoE-knockout mice were fed either low-fat diet (LFD; 5.2% fat, w/w), or Western high fat diet (21.2% fat) containing either no additions (WD), 1.5% alpha-CD (WDA); 1.5% beta-CD (WDB); or 1.5% oligofructose-enriched inulin (WDI). Although plasma lipids were similar after 11 weeks on theWDvs. WDA diets, aortic atherosclerotic lesions were 65% less in mice on WDA compared toWD (P < 0.05), and similar tomice fed the LFD. No effect on atherosclerosis was observed for the other WD supplemented diets. By RNA-seq analysis of 16S rRNA, addition of alpha-CD to the WD resulted in significantly decreased cecal bacterial counts in genera Clostridium and Turicibacterium, and significantly increased Dehalobacteriaceae. At family level, Comamonadaceae significantly increased and Peptostreptococcaceae showed a negative trend. Several of these bacterial count changes correlated negatively with % atherosclerotic lesion and were associated with increased cecum weight and decreased plasma cholesterol levels. Conclusion: Addition of alpha-CD to the diet of apoE-knockoutmice decreases atherosclerosis and is associated with changes in the gut flora. |
| Rights: | This is the peer reviewed version of the following article:
Toshihiro Sakurai, Akiko Sakurai, Ye Chen, Boris L. Vaisman, Marcelo J. Amar, Milton Pryor, Seth G. Thacker, Xue Zhang, Xujing Wang, Yubo Zhang, Jun Zhu, Zhi-Hong Yang, Lita A. Freeman, Alan T. Remaley (2017), Dietary alpha-cyclodextrin reduces atherosclerosis and modifies gut flora in apolipoprotein E-deficient mice. Molecular nutrition & food research, 61(8): 1600804., which has been published in final form at 10.1002/mnfr.201600804. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/71308 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 櫻井 俊宏
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