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Hokkaido University Collection of Scholarly and Academic Papers >
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Multipoint fluorescence correlation spectroscopy using spatial light modulator
| Title: | Multipoint fluorescence correlation spectroscopy using spatial light modulator |
| Authors: | Yamamoto, Johtaro Browse this author →KAKEN DB | | Mikuni, Shintaro Browse this author →KAKEN DB | | Kinjo, Masataka Browse this author →KAKEN DB |
| Issue Date: | 1-Dec-2018 |
| Publisher: | Optical Society of America |
| Journal Title: | Biomedical Optics Express |
| Volume: | 9 |
| Issue: | 12 |
| Start Page: | 5881 |
| End Page: | 5890 |
| Publisher DOI: | 10.1364/BOE.9.005881 |
| Abstract: | A multipoint holographic fluorescence correlation spectroscope (MP-hFCS) was successfully developed. The validity of the MP-hFCS was demonstrated using diffusion measurements of fluorescent dye solutions and of fluorescent proteins in single cells. Furthermore, the successful detection of the nuclear transport of a green fluorescent protein-tagged glucocorticoid receptor a indicates the possibility of being able to monitor directional molecular transport using the MP-hFCS. This allows multipoint analysis of the intermolecular interactions and molecular transport in living cells. Finally, the MP-hFCS can achieve multipoint diffusion measurements with high spatial and time resolution while maintaining a high photon detection sensitivity. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
| Rights: | ©2018 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/74923 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 金城 政孝
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