|
|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
Heavy Water (D2O) Containing Preservation Solution Reduces Hepatic Cold Preservation and Reperfusion Injury in an Isolated Perfused Rat Liver (IPRL) Model
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Heavy Water (D2O) Containing Preservation Solution Reduces Hepatic Cold Preservation and Reperfusion Injury in an Isolated Perfused Rat Liver (IPRL) Model |
| Authors: | Shimada, Shingo Browse this author | | Fukai, Moto Browse this author →KAKEN DB | | Shibata, Kengo Browse this author | | Sakamoto, Sodai Browse this author | | Wakayama, Kenji Browse this author →KAKEN DB | | Ishikawa, Takahisa Browse this author | | Kawamura, Norio Browse this author →KAKEN DB | | Fujiyoshi, Masato Browse this author | | Shimamura, Tsuyoshi Browse this author →KAKEN DB | | Taketomi, Akinobu Browse this author →KAKEN DB |
| Keywords: | heavy water | | D2O | | liver | | cold preservation | | mitochondria | | cytoskeleton |
| Issue Date: | Nov-2019 |
| Publisher: | MDPI |
| Journal Title: | Journal of clinical medicine |
| Volume: | 8 |
| Issue: | 11 |
| Start Page: | 1818 |
| Publisher DOI: | 10.3390/jcm8111818 |
| Abstract: | Background: Heavy water (D2O) has many biological effects due to the isotope effect of deuterium. We previously reported the efficacy of D2O containing solution (Dsol) in the cold preservation of rat hearts. Here, we evaluated whether Dsol reduced hepatic cold preservation and reperfusion injury. Methods: Rat livers were subjected to 48-hour cold storage in University of Wisconsin (UW) solution or Dsol, and subsequently reperfused on an isolated perfused rat liver. Graft function, injury, perfusion kinetics, oxidative stress, and cytoskeletal integrity were assessed. Results: In the UW group, severe ischemia and reperfusion injury (IRI) was shown by histopathology, higher liver enzymes leakage, portal resistance, and apoptotic index, oxygen consumption, less bile production, energy charge, and reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio (versus control). The Dsol group showed that these injuries were significantly ameliorated (versus the UW group). Furthermore, cytoskeletal derangement was progressed in the UW group, as shown by less degradation of alpha-Fodrin and by the inactivation of the actin depolymerization pathway, whereas these changes were significantly suppressed in the Dsol group. Conclusion: Dsol reduced hepatic IRI after extended cold preservation and subsequent reperfusion. The protection was primarily due to the maintenance of mitochondrial function, cytoskeletal integrity, leading to limiting oxidative stress, apoptosis, and necrosis pathways. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/76618 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
