| Title: | Isotope Microscopic Observation of Osteogenesis Process Forming Robust Bonding of Double Network Hydrogel to Bone |
| Authors: | Nonoyama, Takayuki Browse this author →KAKEN DB |
| Wang, Lei Browse this author →KAKEN DB |
| Tsuda, Masumi Browse this author →KAKEN DB |
| Suzuki, Yuki Browse this author |
| Kiyama, Ryuji Browse this author |
| Yasuda, Kazunori Browse this author →KAKEN DB |
| Tanaka, Shinya Browse this author →KAKEN DB |
| Nagata, Kousuke Browse this author |
| Fujita, Ryosuke Browse this author |
| Sakamoto, Naoya Browse this author →KAKEN DB |
| Kawasaki, Noriyuki Browse this author →KAKEN DB |
| Yurimoto, Hisayoshi Browse this author →KAKEN DB |
| Gong, Jian Ping Browse this author →KAKEN DB |
| Keywords: | bone absorbable materials |
| hydroxyapatite |
| isotope microscopy |
| osteogenesis |
| tough hydrogels |
| Issue Date: | 3-Feb-2021 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Advanced Healthcare Materials |
| Volume: | 10 |
| Issue: | 3 |
| Start Page: | 2001731 |
| Publisher DOI: | 10.1002/adhm.202001731 |
| Abstract: | Tough double network (DN) hydrogels are promising substitutes of soft supporting tissues such as cartilage and ligaments. For such applications, it is indispensable to robustly fix the hydrogels to bones with medically feasible methods. Recently, robustly bonding the DN hydrogels to defected bones of rabbits in vivo has been proved successful. The low crystalline hydroxyapatite (HAp) of calcium-phosphate-hydroxide salt coated on the surface layer of the DN hydrogels induced spontaneous osteogenesis penetrating into the semi-permeable hydrogels to form a gel/bone composite layer. In this work, the Ca-44 isotope-doped HAp/DN hydrogel is implanted in a defect of rabbit femoral bone and the dynamic osteogenesis process at the gel/bone interface is analyzed by tracing the calcium isotope ratio using isotope microscopy. The synthetic HAp hybridized on the surface layer of DN gel dissolves rapidly in the first two weeks by inflammation, and then the immature bone with a gradient structure starts to form in the gel region, reutilizing the dissolved Ca ions. These results reveal, for the first time, that synthetic HAp is reutilized for osteogenesis. These facts help to understand the lifetime of bone absorbable materials and to elucidate the mechanism of spontaneous, non-toxic, but strong fixation of hydrogels to bones. |
| Rights: | This is the peer reviewed version of the following article: Isotope Microscopic Observation of Osteogenesis Process Forming Robust Bonding of Double Network Hydrogel to Bone, which has been published in final form at https://doi.org/10.1002/adhm.202001731. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/84005 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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