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Enhancement of interleukin-2 production by bovine peripheral blood mononuclear cells treated with the combination of anti-programmed death-ligand 1 and cytotoxic T lymphocyte antigen 4 chimeric monoclonal antibodies
Title: | Enhancement of interleukin-2 production by bovine peripheral blood mononuclear cells treated with the combination of anti-programmed death-ligand 1 and cytotoxic T lymphocyte antigen 4 chimeric monoclonal antibodies |
Authors: | Watari, Kei Browse this author | Konnai, Satoru Browse this author →KAKEN DB | Okagawa, Tomohiro Browse this author →KAKEN DB | Maekawa, Naoya Browse this author →KAKEN DB | Sajiki, Yamato Browse this author | Kato, Yukinari Browse this author | Suzuki, Yasuhiko Browse this author →KAKEN DB | Murata, Shiro Browse this author →KAKEN DB | Ohashi, Kazuhiko Browse this author →KAKEN DB |
Keywords: | cattle | chimeric antibody | cytotoxicT lymphocyte antigen 4 | interleukin-2 | programmed death-ligand 1 |
Issue Date: | 18-Nov-2021 |
Publisher: | 公益社団法人 日本獣医学会 (The Japanese Society of Veterinary Science) |
Journal Title: | Journal of veterinary medical science |
Volume: | 84 |
Issue: | 1 |
Start Page: | 6 |
End Page: | 15 |
Publisher DOI: | 10.1292/jvms.21-0552 |
Abstract: | Our previous studies demonstrate the therapeutic efficacy against bovine diseases of an anti-bovine programmed death-ligand 1 (PD-L1) chimeric antibody. In humans, PD-1 and PD-L1 antibodies are more effective when combined with an antibody targeting cytotoxic T lymphocyte antigen 4 (CTLA-4) and these combination therapies are therefore clinically used. Here we generated an anti-bovine CTLA-4 chimeric antibody (chAb) to enhance the therapeutic efficacy of the PD-L1 antibody. We further analyzed the effects of dual blockade of CTLA-4 and PD-1 pathways on T-cell responses. The established anti-bovine CTLA-4 chAb showed comparable blocking activity on the binding of bovine CTLA-4 to CD80 and CD86 as the anti-bovine CTLA-4 mouse monoclonal antibody. Anti-bovine CTLA-4 chAb also significantly increased IL-2 production from bovine peripheral blood mononuclear cells (PBMCs). Further, the combination of anti-CTLA-4 chAb with anti-PD-L1 chAb significantly upregulated IL-2 production by PBMCs. These results suggest that the combination of antibodies have higher potential to enhance immune responses against pathogens compared with single administration. |
Type: | article |
URI: | http://hdl.handle.net/2115/84735 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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