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TSS-seq of Toxoplasma gondii sporozoites revealed a novel motif in stage-specific promoters
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Title: | TSS-seq of Toxoplasma gondii sporozoites revealed a novel motif in stage-specific promoters |
Authors: | Kidaka, Taishi Browse this author | Sugi, Tatsuki Browse this author | Hayashida, Kyoko Browse this author | Suzuki, Yutaka Browse this author | Xuan, Xuenan Browse this author | Dubey, Jitender P. Browse this author | Yamagishi, Junya Browse this author →KAKEN DB |
Keywords: | Toxoplasma gondii | Sporozoites | Transcriptome | Transcriptional start sites | cis-elements |
Issue Date: | Mar-2022 |
Publisher: | Elsevier |
Journal Title: | Infection, Genetics and Evolution |
Volume: | 98 |
Start Page: | 105213 |
Publisher DOI: | 10.1016/j.meegid.2022.105213 |
Abstract: | Toxoplasma gondii is one of the most common zoonotic protozoan parasites. It has three major infectious stages: rapidly multiplying tachyzoites (Tz), slowly replicating bradyzoites (Bz) and a resting/free-living stage, sporo-zoites (Sz). The regulatory mechanisms governing stage-specific gene expression are not fully understood. Few transcriptional start sites (TSS) are known for Sz. In this study, we obtained TSS of Sz using an oligo-capping method and RNA-seq analysis. We identified 1,043,503 TSS in the Sz transcriptome. These defined 38,973 TSS clusters, of which, 11,925 were expressed in Sz and 1535 TSS differentially expressed in Sz. Based on these data, we defined promoter regions and novel sporozoite stage-specific motifs using MEME. TGTANNTACA was distributed around-55 to-75 regions from each TSS. Interestingly, the same motif was reported in another apicomplexan, Plasmodium berghei, as a cis-element of female-specific gametocyte genes, implying the presence of common regulatory machinery. Further comparative analysis should better define the distribution and function of these elements in other members of this important parasitic phylum. |
Rights: | https://creativecommons.org/licenses/by/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/85615 |
Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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