|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Interfacial biosilica coating of chitosan gel using fusion silicatein to fabricate robust hybrid material for biomolecular applications
| Title: | Interfacial biosilica coating of chitosan gel using fusion silicatein to fabricate robust hybrid material for biomolecular applications |
| Authors: | Godigamuwa, Kasun Browse this author | | Nakashima, Kazunori Browse this author | | Tsujitani, Sota Browse this author | | Naota, Ryo Browse this author | | Maulidin, Ilham Browse this author | | Kawasaki, Satoru Browse this author →KAKEN DB |
| Issue Date: | 28-Feb-2023 |
| Publisher: | Royal Society of Chemistry |
| Journal Title: | Journal of materials chemistry B |
| Volume: | 11 |
| Issue: | 8 |
| Start Page: | 1654 |
| End Page: | 1658 |
| Publisher DOI: | 10.1039/d2tb02581g |
| Abstract: | An enzyme-encapsulated silica-based hybrid material was developed using a chitosan gel. Fusion silicatein (InaKC-ChBD-Sil), silicatein fused with a soluble tag and chitin-binding domain, was employed as an interfacial catalyst to form silica on a chitosan gel matrix under physiological conditions, and horseradish peroxidase was immobilised on the hybrid material. Silica formation on the gel was verified via fluorescence microscopy using a designed fusion protein called TBP-mCherry, a fluorescent protein fused with a silica-binding peptide. We report a chitosan gel-silica hybrid material capable of encapsulating enzymes for biomedical and environmental applications. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/88579 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
