Contribution to a conference proceedings/Journal Article

FZJ-2016-00485

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes

Pilas, J. ; Mariano, K. ; Keusgen, M. ; Selmer, T. ; Schöning, M. J. (Corresponding author)FZJ*

2015
Elsevier Amsterdam [u.a.]

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Please use a persistent id in citations: http://hdl.handle.net/2128/9781  doi:10.1016/j.proeng.2015.08.702

Abstract: An enzyme-based multi-parameter sensor chip for the simultaneous measurement of formate, D- and L-lactate is presented. Thereby, the combination of a diaphorase (DIA) from Clostridium kluyveri with different NAD+-dependent dehydrogenases enables the specific recognition of each compound. The amperometric detection is performed via monitoring of the oxidation current of enzymatically produced hexacyanoferrate(II) at +300 mV vs. Ag/AgCl. Chemical cross-linking with glutaraldehyde is used for enzyme immobilization. Thereby, the sensor signal is investigated at different concentrations of the cross-linker in the enzyme membrane. The obtained results indicate that by optimization of the immobilization matrix, the sensor performance can be improved.

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Contributing Institute(s):

1. Bioelektronik (PGI-8)

Research Program(s):

1. 523 - Controlling Configuration-Based Phenomena (POF3-523) (POF3-523)

Appears in the scientific report 2015

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