Factors affecting transposition of the Himar1 mariner transposon in vitro
Lampe, David J.; Grant, Theresa E.; Robertson, Hugh M.
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https://hdl.handle.net/2142/222
Description
Title
Factors affecting transposition of the Himar1 mariner transposon in vitro
Author(s)
Lampe, David J.
Grant, Theresa E.
Robertson, Hugh M.
Issue Date
1998-05
Keyword(s)
Genetics
Abstract
"Mariner family transposable elements are widespread in animals, but their regulation is poorly understood, partly because only two are known to be functional. These are particular copies of the Dmmar1
element from Drosophila mauritiana, for example, Mos1, and the consensus sequence of the Himar1 element from the horn fly, Haematobia irritans. An in vitro transposition system was refined to investigate several parameters that influence the transposition of Himar1. Transposition products accumulated linearly over a period of 6 hr. Transposition frequency increased with temperature and was dependent on Mg21
concentration. Transposition frequency peaked over a narrow range of transposase concentration. The decline at higher concentrations, a phenomenon observed in vivo with Mos1, supports the suggestion that
mariners may be regulated in part by ""overproduction inhibition."" Transposition frequency decreased exponentially with increasing transposon size and was affected by the sequence of the flanking DNA of
the donor site. A noticeable bias in target site usage suggests a preference for insertion into bent or bendable DNA sequences rather than any specific nucleotide sequences beyond the TA target site."
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