Development and Evaluation of a Two-Step Membrane Filter Method for Fecal Coliform Recovery in Chlorinated Sewage Effluents

Lin, S.D.

Permalink

https://hdl.handle.net/2142/77797 Copy

Description

Title

Development and Evaluation of a Two-Step Membrane Filter Method for Fecal Coliform Recovery in Chlorinated Sewage Effluents

Author(s)

Lin, S.D.

Issue Date

1978

Keyword(s)

• fecal coliform
• membrane comparison
• multiple-tube (MPN) method
• two-step membrane filtebacteria analysis
• chlorinated wastewater effluents
• Duncan's multiple rr method
• bacteria analysis
• Duncan's multiple range test

Abstract

A membrane filter (MF) method for fecal coliform (FC) in chlorinated wastewater effluents was developed and evaluated with 10 different types of MFs. A membrane is transferred onto M-FC agar for incubation at 44.5°C for 18±2 hours after pre-enrichment with phenol red lactose broth and incubation at 35°C for 4 hours. Results of 126 comparisons indicated that the FC recoveries by this two-step MF method were comparable to those obtained by the multiple tube (MPN) procedure. The results of comparisons of 10 MF types for FC recovery in chlorinated effluents from five plants with the two-step MF procedure were analyzed by Duncan's multiple range test. The FC recovery rates of the MFs tested can be ranked as follows: Millipore HC ~ Gelman GN-6 ~ Sartorius green > Helena Titan GH ~ Nuclepore N040 ~ Sartorius SM-137 56 ~ Schleicher & Schuell BC 07 > Johns-Manville Sterilized ~ S & S B-9 > J-M Radiation.

Publisher

Illinois State Water Survey

Series/Report Name or Number

Report of Investigation (Illinois State Water Survey) RI-087

Type of Resource

text

Language

en

Permalink

http://hdl.handle.net/2142/77797

Copyright and License Information

Copyright, University of Illinois Board of Trustees. All rights reserved. This document is a product of the Illinois State Water Survey, and has been selected and made available by the Illinois State Water Survey and the University Library, University of Illinois at Urbana-Champaign. It is intended for research and educational use, and proper attribution is requested.

Owning Collections