Endotoxins are potentially toxic natural compounds that are found in the outer membranes of various gram-negative bacteria. Endotoxins are in large part responsible for dramatic clinical manifestations of infections with pathogenic gram-negative bacteria. In order to develop a sensor for endotoxin that would not require special training and could withstand the conditions of an industrial or medical setting, we have used a method called in-vitro selection or Systematic Evolution of Ligands by Exponential Enrichment (SELEX) to obtain DNA aptamers with high specificity and selectivity for endotoxin. While we were able to confirm activity from the selection, we were unable to identify the active DNA. Given further work, a colorimetric or fluorophore-based sensor should be able to be developed based upon the DNA aptamers that have been isolated.
Publisher
Champaign, IL : Illinois Sustainable Technology Center
Series/Report Name or Number
RR Series (Illinois Sustainable Technology Center) ; 123
Type of Resource
text
Language
en
Permalink
http://hdl.handle.net/2142/78016
Sponsor(s)/Grant Number(s)
Illinois Sustainable Technology Center (Grant No. HWR09217)
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
