Noise in noise out - single molecule studies of molecular motors

Tjioe, Marco

Permalink

https://hdl.handle.net/2142/98156 Copy

Description

Title

Noise in noise out - single molecule studies of molecular motors

Author(s)

Tjioe, Marco

Issue Date

2017-05-26

Director of Research (if dissertation) or Advisor (if thesis)

Selvin, Paul R.

Doctoral Committee Chair(s)

Selvin, Paul R.

Committee Member(s)

• Chemla, Yann R.
• DeMarco, Brian L.
• Zhang, Kai

Department of Study

School of Molecular & Cell Bio

Discipline

Biophysics & Computnl Biology

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

• Single molecule
• Kinesin
• Dynein
• Noise
• Frames per step (FPS)
• Magnetic purification
• Signal-to-noise ratio
• Molecular motor

Abstract

Many cellular functions rely on the movements of molecular motors. Single molecule studies of these motors allow investigation into their step-sizes and stall forces. Such studies inevitably involve noise, which is inherent in the biological and imaging systems probing these motors. Having to work with noise in the system, we develop a parameter, called the FIONA Index, which serves as a guide for data quality based on noise per step-size (NPS) and frames per step (FPS). Attempting to bring the noise out of the system, we develop Magnetic Cytoskeleton Affinity (MiCA) purification to remove excess dyes that prevent clear observation of individual motors. We show that with appropriate treatment of noise, complex systems can be assembled to study how multiple motors work together.

Graduation Semester

2017-08

Type of Resource

text

Permalink

http://hdl.handle.net/2142/98156

Copyright and License Information

Copyright 2017 Marco Tjioe

Owning Collections