In this work, an electrochemical DNA aptasensor for acetamiprid is presented. The aptasensor is based on a dual signal amplified strategy by employing a gold-polyaniline modified screen-printed electrode as sensor platform and an enzyme-linked label for sensitive detection. The polyaniline-gold nanocomposite modified electrodes were firstly modified with a mixed monolayer of a thiol-tethered DNA aptamer and 6-mercapto-1-hexanol. The acetamiprid solution was then incubated with the aptasensor. An enzyme-amplified detection scheme, based on the coupling of a streptavidin-alkaline phosphatase conjugate and secondary biotinylated aptamer was then applied. The enzyme catalyzed the hydrolysis of the electroinactive 1-naphthyl-phosphate to 1-naphthol. This electroactive product was detected by means of differential pulse voltammetry. A calibration curve between 0-200 nM acetamiprid concentration range was obtained.

Recent progress in biosensors for acetemiprid detection / Marrazza, Giovanna; Rapini, Riccardo;. - STAMPA. - (2016), pp. 58-64. (Intervento presentato al convegno 5th Workshop Specific methods for food safety and quality tenutosi a Belgrade, Serbia nel September 27th 2016).

Recent progress in biosensors for acetemiprid detection

MARRAZZA, GIOVANNA;RAPINI, RICCARDO
2016

Abstract

In this work, an electrochemical DNA aptasensor for acetamiprid is presented. The aptasensor is based on a dual signal amplified strategy by employing a gold-polyaniline modified screen-printed electrode as sensor platform and an enzyme-linked label for sensitive detection. The polyaniline-gold nanocomposite modified electrodes were firstly modified with a mixed monolayer of a thiol-tethered DNA aptamer and 6-mercapto-1-hexanol. The acetamiprid solution was then incubated with the aptasensor. An enzyme-amplified detection scheme, based on the coupling of a streptavidin-alkaline phosphatase conjugate and secondary biotinylated aptamer was then applied. The enzyme catalyzed the hydrolysis of the electroinactive 1-naphthyl-phosphate to 1-naphthol. This electroactive product was detected by means of differential pulse voltammetry. A calibration curve between 0-200 nM acetamiprid concentration range was obtained.
2016
5th Workshop Specific methods for food safety and quality
5th Workshop Specific methods for food safety and quality
Belgrade, Serbia
September 27th 2016
Marrazza, Giovanna; Rapini, Riccardo;
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1057237
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