This work presents Raman spectra obtained from thin films of protein materials which are commonly used as binding media in painted works of art. Spectra were recorded over the spectral range of 3250-250 cm(-1), using an excitation wavelength of 785 nm, and several bands have been identified in the fingerprint region that correspond to the various proteins examined. Differences in the C-H vibrations located between 3200 and 2700 cm(-1) can be accounted for with reference to the amino acid composition of the protein-based binding media as well as the presence of fatty acid esters, in the case of egg yolk. In addition, the discrimination of different proteins on the basis of variations in spectra between 3200 and 2700 cm(-1) can be achieved following multivariate analysis of a large data set of spectra, providing a novel and nondestructive alternative based on Raman spectroscopy to other methods commonly used for the analysis of proteins.
Raman Spectra of Proteinaceous Materials Used in Paintings: A Multivariate Analytical Approach for Classification and Identification / AUSTIN NEVIN; IACOPO OSTICIOLI; DEMETRIOS ANGLOS; AVIVA BURNSTOCK; SHARON CATHER; E. CASTELLUCCI. - In: ANALYTICAL CHEMISTRY. - ISSN 0003-2700. - STAMPA. - 79 (16):(2007), pp. 6143-6151. [10.1021/ac070373j]
Raman Spectra of Proteinaceous Materials Used in Paintings: A Multivariate Analytical Approach for Classification and Identification
CASTELLUCCI, EMILIO MARIO
2007
Abstract
This work presents Raman spectra obtained from thin films of protein materials which are commonly used as binding media in painted works of art. Spectra were recorded over the spectral range of 3250-250 cm(-1), using an excitation wavelength of 785 nm, and several bands have been identified in the fingerprint region that correspond to the various proteins examined. Differences in the C-H vibrations located between 3200 and 2700 cm(-1) can be accounted for with reference to the amino acid composition of the protein-based binding media as well as the presence of fatty acid esters, in the case of egg yolk. In addition, the discrimination of different proteins on the basis of variations in spectra between 3200 and 2700 cm(-1) can be achieved following multivariate analysis of a large data set of spectra, providing a novel and nondestructive alternative based on Raman spectroscopy to other methods commonly used for the analysis of proteins.I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.