Almost complete assignment (97%) of NMR resonances was obtained for the reduced, Cu(I), form of prokaryotic CuZnSOD from Salmonella enterica. C-13 direct detection was used to complement the standard bouquet o(f) H-1 detected triple resonance experiments and contributed to the identification of proline backbone resonances and to side chains assignments of Asx, Glx and aromatic rings. This is the only complete assignment available for monomer SOD from prokaryotic organisms.
NMR Assignment of Reduced form of Copper, Zinc Superoxide Dismutase from Salmonella enterica / B. JIMENEZ; M. MORI; A. BATTISTONI; M. SETTE; M. PICCIOLI. - In: BIOMOLECULAR NMR ASSIGNMENTS. - ISSN 1874-2718. - STAMPA. - 1:(2007), pp. 65-67. [10.1007/s12104-007-9017-0]
NMR Assignment of Reduced form of Copper, Zinc Superoxide Dismutase from Salmonella enterica
MORI, MIRKO;PICCIOLI, MARIO
2007
Abstract
Almost complete assignment (97%) of NMR resonances was obtained for the reduced, Cu(I), form of prokaryotic CuZnSOD from Salmonella enterica. C-13 direct detection was used to complement the standard bouquet o(f) H-1 detected triple resonance experiments and contributed to the identification of proline backbone resonances and to side chains assignments of Asx, Glx and aromatic rings. This is the only complete assignment available for monomer SOD from prokaryotic organisms.
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