We compared the binding properties of [3H]-nitrendipine in heart membranes from normal guinea-pig heart and from hypoxic or hypoxic and reoxygenated heart. The [3H]-nitrendipine binds a single class of high capacity (Bmax 667.2 +/- 105.2) with high affinity (KD 0.14 +/- 0.02) binding sites. By contrast, in membranes of hypoxic and reoxygenated heart the Bmax decreases significantly while it remains unaffected during hypoxia. Xanthinoxidase activity is increased in hypoxic-reoxygenated hearts.
[3H]-nitrendipine binding in membranes obtained from hypoxic and reoxygenated heart / Matucci R; Bennardini F; Sciammarella ML; Baccaro C; Stendardi I; Franconi F; Giotti A.. - In: BIOCHEMICAL PHARMACOLOGY. - ISSN 0006-2952. - STAMPA. - 36:(1987), pp. 1059-1062.
[3H]-nitrendipine binding in membranes obtained from hypoxic and reoxygenated heart.
MATUCCI, ROSANNA;STENDARDI, ISABELLA;FRANCONI, FLAVIA;GIOTTI, ALBERTO
1987
Abstract
We compared the binding properties of [3H]-nitrendipine in heart membranes from normal guinea-pig heart and from hypoxic or hypoxic and reoxygenated heart. The [3H]-nitrendipine binds a single class of high capacity (Bmax 667.2 +/- 105.2) with high affinity (KD 0.14 +/- 0.02) binding sites. By contrast, in membranes of hypoxic and reoxygenated heart the Bmax decreases significantly while it remains unaffected during hypoxia. Xanthinoxidase activity is increased in hypoxic-reoxygenated hearts.
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