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Improved protocol for the isolation of naïve follicular dendritic cells
http://hdl.handle.net/2241/00144540
http://hdl.handle.net/2241/0014454030f58f10-0256-4609-8fe9-ae22ec04edb9
名前 / ファイル | ライセンス | アクション |
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MI_78 (1.9 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2016-12-15 | |||||
タイトル | ||||||
タイトル | Improved protocol for the isolation of naïve follicular dendritic cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Sato, Kazuki
× Sato, Kazuki× Honda, Shin-ichiro× Shibuya, Akira× Shibuya, Kazuko |
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著者別名 |
渋谷, 彰
× 渋谷, 彰× 渋谷, 和子 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Follicular dendritic cells (FDCs) in lymphoid organs play an important role in the humoral immune response. However, because the isolation of FDCs is difficult due to their very small population size and fragility under mechanical and chemical stresses, the genetic and biochemical characteristics of FDCs remain unclear. Previously, we identified FDCs as ICAM-1+ cells in the CD45− non-hematopoietic cell fraction from naïve mouse spleen after cell separation by means of digestion with a combination of enzymes. In the present study, using a new combination of enzymes, we found that FDCs are highly enriched in the CD45−ICAM-1+CD21/35+ cell fraction. CD45−ICAM-1+CD21/35+ cells in the mouse spleen retained an antigen administered in vivo for more than 7 days. Moreover, CD45−ICAM-1+CD21/35+ cells isolated from the spleen of mice administered with a cognate antigen enhanced the survival and proliferation of antigen-specific B cells in vitro. Our improved protocol for the isolation of naïve FDCs will be useful for the analysis of FDCs in vitro and in vivo. | |||||
書誌情報 |
Molecular immunology 巻 78, p. 140-145, 発行日 2016-10 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0161-5890 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00307483 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 27639061 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.molimm.2016.09.011 | |||||
権利 | ||||||
権利情報 | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Elsevier |