WEKO3
アイテム
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KOnezumi: a web application for automating gene disruption strategies to generate knockout mice
http://hdl.handle.net/2241/00159327
http://hdl.handle.net/2241/00159327d181022f-cbd8-44e2-86b8-9e87d2c92485
名前 / ファイル | ライセンス | アクション |
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Bioinformatics_35-18 (308.6 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2020-01-20 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | KOnezumi: a web application for automating gene disruption strategies to generate knockout mice | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
久野, 朗広
× 久野, 朗広× 水野, 聖哉× 高橋, 智 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A Summary: Although gene editing using the CRISPR/Cas9 system enables the rapid generation of knockout mice, constructing an optimal gene disruption strategy is still labourious. Here, we propose KOnezumi, a simple and user-friendly web application, for use in automating the design of knockout strategies for multiple genes. Users only need to input gene symbols, and then KOnezumi returns target exons, gRNA candidates to delete the target exons, genotyping PCR primers, nucleotide sequences of the target exons and coding sequences of expected deletion products. KOnezumi enables users to easily and rapidly apply a rational strategy to accelerate the generation of KO mice | |||||
書誌情報 |
en : Bioinformatics 巻 35, 号 18, p. 3479-3481, 発行日 2019-09 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1367-4803 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11211127 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 30726877 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1093/bioinformatics/btz090 | |||||
権利 | ||||||
権利情報 | ©The Author(s) 2019. Published by Oxford University Press. | |||||
権利 | ||||||
権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | OXFORD UNIV PRESS |