Molecular analysis of Human Herpes Virus 8 and associated lesions
Citation:
Ivan Silva, 'Molecular analysis of Human Herpes Virus 8 and associated lesions', [thesis], Trinity College (Dublin, Ireland). School of Medicine. Discipline of Histopathology & Morbid Anatomy, 2003, pp 443Download Item:
Silva TCD THESIS 7382 Molecular analysis.pdf (PDF) 253.6Mb
Abstract:
This thesis looks at the bio-pathways of HHV8 through the transformation of endothelial cell lines. This was performed in two separate but mutually contributing experiments. First, endothelial cell lines were infected using purified HHV8 virus, which was obtained
from cultured BC-3 cell lines, derived from an effusion lymphoma. As a follow up, HUVEC and BC-3 cell lines were placed in the same flask to see if there was transmission of the virus from intact BC-3 cells to HUVECS. RNA was extracted from infected HU VECs and transcription of v-cyclin was quantified by TaqM an PCR. The
HHV8 infected endothelial cells were found to contain 50-100 copies per 100 ng of template in both uninduced and induced cells. Three genes: vIL-6, LANA and GPCR
could be amplified from cDNAs of infected HUVECs. Viral IL-6 transcription was positive only in the TPA treated cells. Orf-73 (LANA) and O rf-74 (GPCR) transcripts
were detected with and without TPA inductions.
Author: Silva, Ivan
Advisor:
O'Leary, JohnQualification name:
Doctor of Philosophy (Ph.D.)Publisher:
Trinity College (Dublin, Ireland). School of Medicine. Discipline of Histopathology & Morbid AnatomyNote:
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