[en] Objectives: To evaluate quantitatively cytokeratin and vimentin staining in glandular and stromal cells of eutopic and ectopic endometrium.
DESIGN:
The investigation of cytokeratin and vimentin was carried out using the new computerized technology of image analysis.
SETTING:
University Hospital, Department of Gynecology.
PATIENTS:
Biopsies were taken from patients undergoing a laparoscopy for infertility (29 endometrial biopsies and 31 biopsies of peritoneal endometriosis). None of them were treated.
RESULTS:
Cyclic variations of cytokeratin and vimentin staining were noted in eutopic endometrium. The cytokeratin and vimentin staining pattern consistently was lower in ectopic endometrium than in eutopic endometrium.
CONCLUSIONS:
Endometriotic epithelial and stromal cells undergo a complex program of differentiation giving them histochemical characteristics similar to those observed in endometrium. Such a concomitant expression of antigenicity indicates their close relationship with their mesodermal müllerian origin.
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
NISOLLE, Michelle ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Casanas-Roux, Françoise
Donnez, Jacques
Language :
English
Title :
Coexpression of cytokeratin and vimentin in eutopic endometrium and endometriosis throughout the menstrual cycle: evaluation by a computerized method.
M.A. McNutt J.W. Bolen A.M. Gown S.P. Hammar A.M. Vogel Coexpression of intermediate filaments in human epithelial neoplasms Ultrastruct Pathol 9 1985 31 43
G. Viale M. Gambacorta P. Dell’Orto G. Coggi Coexpression of cytokeratins and vimentin in common epithelial tumours of the ovary: an immunocytochemical study of eighty-three cases Virchows Arch [A] 413 1988 91 101
R.F.P.M. Kruitwagen L.G. Poels W.N.P. Willemsen P.H.K. Jap I.J.Y. de Ronde T.G.J.M. Hanselaar Immunocytochemical marker profile of endometriotic epithelial, endometrial epithelial, and mesothelial cells: a comparative study Eur J Obstet Gynecol Reprod Biol 41 1991 215 223
S. Tabibzadeh Distinct subsets of stromal cells confined to unique microenvironments in human endometrium throughout the menstrual cycle Am J Reprod Immunol 26 1991 5 10
C.J. Matthews C.P.F. Redfern B.H. Hirst E.J. Thomas Characterization of human purified epithelial and stromal cells from endometrium and endometriosis in tissue culture Fertil Steril 57 1992 990 997
M. Ohwada M. Suzuki T. Tamada Immunohistochemical investigation of vimentin of uterine endometrial and endocervical adenocarcinoma Nippon Sanka Fujinka Gakkai Zasshi 42 1990 1237 1243
E.R. Norwitz S. Fernandez-Shaw D.H. Barlow P.M. Starkey Expression of intermediate filament in endometrial glands changes with the onset of pregnancy and in endometriosis Hum Reprod 6 1991 1470 1473
M. Nakamura H. Katabuchi T. Tohya Y. Fukumatsu K. Matsu-ura H. Okamura Scanning electron microscopic and immunohistochemical studies of pelvic endometriosis Hum Reprod 8 1993 2218 2226
R.W. Noyes A.T. Hertig J. Rock Dating the endometrial biopsy Fertil Steril 1 1950 3 15
K.S. McCarty Jr L.S. Miller E.B. Cox J. Konrath K.S. McCarty Sr Estrogen receptor analysis: correlation of biochemical and immunohistochemical methods using monoclonal anti-receptor antibodies Arch Pathol Lab Med 109 1985 716 721
R. Moll R. Levy B. Czernobilsky T.P. Hohlweg-Majer G. Dallenbach-Hellweg W.W. Franke Cytokeratins of normal epithelia and some neoplasms of the female genital tract Lab Invest 49 1983 599 610
I.Z. Glatstein Y.M. Choi R. Osathanondh J. Yeh Human fetal uterine cells: culture, characterization, and analysis of growth factor receptor gene expression J Clin Endocrinol Metab 79 1994 126 133
M. Nisolle F. Casanas-Roux C. Wyns Y. de Menten P-E. Mathieu J. Donnez Immunohistochemical analysis of estrogen and progesterone receptors in endometrium and peritoneal endometriosis: a new quantitative method Fertil Steril 62 1994 751 759
L. Nakopoulou D. Minaretzia C. Tsionou M. Mastrominas Value of immunohistochemical demonstration of several epithelial markers in hyperplasia and neoplastic endometrium Gynecol Oncol 37 1990 346 353