Article (Scientific journals)
Studying RNA-Protein Interactions In Vivo By RNA Immunoprecipitation
Selth, L. A.; Close, Pierre; Svejstrup, J. Q.
2011In Methods in Molecular Biology, 791, p. 253-264
Peer Reviewed verified by ORBi
 

Files


Full Text
Selth et al_MMB 2011.pdf
Publisher postprint (989.82 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
RNA immunoprécipitation; transcription; RNA binding protein
Abstract :
[en] The crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Selth, L. A.
Close, Pierre  ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Svejstrup, J. Q.
Language :
English
Title :
Studying RNA-Protein Interactions In Vivo By RNA Immunoprecipitation
Publication date :
2011
Journal title :
Methods in Molecular Biology
ISSN :
1064-3745
eISSN :
1940-6029
Publisher :
Humana Press, Totowa, United States - New Jersey
Volume :
791
Pages :
253-264
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 08 February 2012

Statistics


Number of views
93 (8 by ULiège)
Number of downloads
4 (4 by ULiège)

Scopus citations®
 
22
Scopus citations®
without self-citations
21
OpenCitations
 
20

Bibliography


Similar publications



Contact ORBi