mass spectrometry; MALDI; MELDI; internal energy; benzylpyridinium
Abstract :
[en] This work describes a method to use relative fragmentation yields to characterize the internal energy distribution of ions produced by matrix-enhanced laser desorption/ionization mass spectrometry (MELDI-MS, see: Wright LG, Cooks RG, Wood KL. Biomed. Mass Spectrom. 1985; 12:153162). Assuming that the fragmentation proceeds statistically and that the collisions in the source lead to a Boltzmann-like distribution of the internal energy, a characteristic parameter, the effective temperature, is introduced to describe the internal energy distribution of the ions observed. The hypotheses, advantages and drawbacks of the implementation of the method that uses substituted benzylpyridinium salts as thermometer ions are discussed. Use is made of two matrices that produce no matrix cations in MELDI and are suitable for small cationic salts. The actual value of this effective temperature significantly depends on an accurate determination of the threshold dissociation energies and on the time spent in the source, in addition to the statistical hypothesis itself. The method could be applied to normalize spectra in order to compare results issued from different instruments.
Research center :
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Disciplines :
Chemistry
Author, co-author :
Greisch, Jean-François ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie physique, spectrométrie de masse
Gabelica, Valérie ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie physique, spectrométrie de masse
Remacle, Françoise ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de chimie physique théorique
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie physique, spectrométrie de masse
Language :
English
Title :
Thermometer ions for matrix-enhanced laser desorption/ionization internal energy calibration
Publication date :
2003
Journal title :
Rapid Communications in Mass Spectrometry
ISSN :
0951-4198
eISSN :
1097-0231
Publisher :
John Wiley & Sons, Inc, Chichester, United Kingdom
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