Article (Scientific journals)
Automated method for the determination of a new matrix metalloproteinase inhibitor in ovine plasma and serum by coupling of restricted access material for on-line sample clean-up to liquid chromatography
Chiap, Patrice; Piette, Marie; Evrard, Brigitte et al.
2005In Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 817 (1), p. 109-117
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Keywords :
matrix metalloproteinase inhibitor; Ro 28-2653; plasma; serum; sample preparation; column-switching; restricted access material; liquid chromatography
Abstract :
[en] A fully automated liquid chromatographic method was developed for the determination of Ro 28-2653, a new synthetic inhibitor of matrix metalloproteinases (MMPs), in ovine serum and plasma. The method was based on the coupling of a pre-column packed with restricted access material, namely LiChrospher RP-8 ADS (alkyl diol silica), for sample clean-up to an analytical column containing octyl silica stationary phase. One hundred mul of biological sample, to which 2-propanol was automatically added, were injected onto the ADS pre-column, which was then washed with a washing liquid consisting of a mixture of 25 mM phosphate buffer (pH 7.0) and acetonitrile (90: 10; v/v) for 10 min. By rotation of the switching valve, the analyte was then eluted in the back-flush mode with the LC mobile phase composed of a mixture of acetonitrile and 25 mM phosphate buffer (pH 7.0) (57:43; v/v). The UV detection was performed at 395 nm. The main parameters likely to influence the sample preparation technique were investigated. The method was then validated over a concentration range from 17.5 to 1950 ng/mI, the first concentration level corresponding to the lower limit of quantitation. At this concentration level, the mean bias and the R.S.D. value for intermediate precision were -2.4% and 4.2%, respectively. (C) 2004 Elsevier B.V. All rights reserved.
Disciplines :
Chemistry
Biochemistry, biophysics & molecular biology
Author, co-author :
Chiap, Patrice ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Piette, Marie ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Evrard, Brigitte  ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Frankenne, Francis
Christiaens, B.
Piel, Géraldine ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique et magistrale
Cataldo, Didier  ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement - Département des sciences biomédicales et précliniques
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Delattre, Luc ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Crommen, Jacques ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Hubert, Philippe  ;  Université de Liège - ULiège > Chimie analytique
Language :
English
Title :
Automated method for the determination of a new matrix metalloproteinase inhibitor in ovine plasma and serum by coupling of restricted access material for on-line sample clean-up to liquid chromatography
Publication date :
05 March 2005
Journal title :
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
ISSN :
1570-0232
eISSN :
1873-376X
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
817
Issue :
1
Pages :
109-117
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 May 2009

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