Human-Phosphate-Binding-Protein inhibits HIV-1 gene transcription and replication.

Cells, Cultured; HIV-1/immunology/physiology; Host-Pathogen Interactions; Humans; Leukocytes, Mononuclear/immunology/virology; Macrophages/immunology/virology; Phosphate-Binding Proteins/immunology; T-Lymphocytes/immunology/virology; Transcription, Genetic; Virus Replication

Abstract :

[en] The Human Phosphate-Binding protein (HPBP) is a serendipitously discovered lipoprotein that binds phosphate with high affinity. HPBP belongs to the DING protein family, involved in various biological processes like cell cycle regulation. We report that HPBP inhibits HIV-1 gene transcription and replication in T cell line, primary peripherical blood lymphocytes and primary macrophages. We show that HPBP is efficient in naive and HIV-1 AZT-resistant strains. Our results revealed HPBP as a new and potent anti HIV molecule that inhibits transcription of the virus, which has not yet been targeted by HAART and therefore opens new strategies in the treatment of HIV infection.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Cherrier, Thomas ; Université de Liège - ULiège > GIGA-Research

Elias, Mikael

Jeudy, Alicia

Gotthard, Guillaume

Le Douce, Valentin

Hallay, Houda

Masson, Patrick

Janossy, Andrea

Candolfi, Ermanno

Rohr, Olivier

Chabriere, Eric

Schwartz, Christian

Language :

English

Title :

Human-Phosphate-Binding-Protein inhibits HIV-1 gene transcription and replication.

Publication date :

2011

Journal title :

Virology Journal

eISSN :

1743-422X

Publisher :

BioMed Central, United Kingdom

Volume :

Pages :

352

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 25 January 2013

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Bibliography

Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Barre-Sinoussi F, Chermann JC, Rey F, Nugeyre MT, Chamaret S, Gruest J, Dauguet C, Axler-Blin C, Vezinet-Brun F, Rouzioux C, et al. Science 1983 220 868 871 10.1126/science.6189183 6189183 (Pubitemid 13080157)
Hide-and-seek: the challenge of viral persistence in HIV-1 infection. Geeraert L, Kraus G, Pomerantz RJ, Annu Rev Med 2008 59 487 501 10.1146/annurev.med.59.062806.123001 17845138 (Pubitemid 351287951)
HIV-1 drug resistance profiles in children and adults with viral load of <50 copies/ml receiving combination therapy. Hermankova M, Ray SC, Ruff C, Powell-Davis M, Ingersoll R, D'Aquila RT, Quinn TC, Siliciano JD, Siliciano RF, Persaud D, Jama 2001 286 196 207 10.1001/jama.286.2.196 11448283 (Pubitemid 32640851)
Genotypic analysis of HIV-1 drug resistance at the limit of detection: virus production without evolution in treated adults with undetectable HIV loads. Kieffer TL, Finucane MM, Nettles RE, Quinn TC, Broman KW, Ray SC, Persaud D, Siliciano RF, J Infect Dis 2004 189 1452 1465 10.1086/382488 15073683 (Pubitemid 38543595)
Intermittent HIV-1 viremia (Blips) and drug resistance in patients receiving HAART. Nettles RE, Kieffer TL, Kwon P, Monie D, Han Y, Parsons T, Cofrancesco J Jr, Gallant JE, Quinn TC, Jackson B, et al. Jama 2005 293 817 829 10.1001/jama.293.7.817 15713771 (Pubitemid 40250365)
Molecular mechanisms of HIV-1 persistence in the monocyte-macrophage lineage. Le Douce V, Herbein G, Rohr O, Schwartz C, Retrovirology 2010 7 32
HIV-1 regulation of latency in the monocyte-macrophage lineage and in CD4+ T lymphocytes. Redel L, Le Douce V, Cherrier T, Marban C, Janossy A, Aunis D, Van Lint C, Rohr O, Schwartz C, J Leukoc Biol 2009
The macrophage in HIV-1 infection: from activation to deactivation? Herbein G, Varin A, Retrovirology 2010 7 33
Macrophages and HIV infection: therapeutical approaches toward this strategic virus reservoir. Aquaro S, Calio R, Balzarini J, Bellocchi MC, Garaci E, Perno CF, Antiviral Res 2002 55 209 225 10.1016/S0166-3542(02)00052-9 12103427 (Pubitemid 34718126)
Relative potency of protease inhibitors in monocytes/macrophages acutely and chronically infected with human immunodeficiency virus. Perno CF, Newcomb FM, Davis DA, Aquaro S, Humphrey RW, Calio R, Yarchoan R, J Infect Dis 1998 178 413 422 9697721 (Pubitemid 28347923)
Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection. Chun TW, Carruth L, Finzi D, Shen X, DiGiuseppe JA, Taylor H, Hermankova M, Chadwick K, Margolick J, Quinn TC, et al. Nature 1997 387 183 188 10.1038/387183a0 9144289 (Pubitemid 27209433)
Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Finzi D, Hermankova M, Pierson T, Carruth LM, Buck C, Chaisson RE, Quinn TC, Chadwick K, Margolick J, Brookmeyer R, et al. Science 1997 278 1295 1300 10.1126/science.278.5341.1295 9360927 (Pubitemid 27495742)
Quantifying residual HIV-1 replication in patients receiving combination antiretroviral therapy. Zhang L, Ramratnam B, Tenner-Racz K, He Y, Vesanen M, Lewin S, Talal A, Racz P, Perelson AS, Korber BT, et al. N Engl J Med 1999 340 1605 1613 10.1056/NEJM199905273402101 10341272 (Pubitemid 29249429)
HIV pharmacology: barriers to the eradication of HIV from the CNS. McGee B, Smith N, Aweeka F, HIV Clin Trials 2006 7 142 153 10.1310/AW2H-TP5C-NP43-K6BY 16880170 (Pubitemid 44309004)
Investigation of distribution, transport and uptake of anti-HIV drugs to the central nervous system. Sawchuk RJ, Yang Z, Adv Drug Deliv Rev 1999 39 5 31 10.1016/S0169-409X(99)00017-4 10837765 (Pubitemid 29430227)
Discrepancies between protease inhibitor concentrations and viral load in reservoirs and sanctuary sites in human immunodeficiency virus-infected patients. Solas C, Lafeuillade A, Halfon P, Chadapaud S, Hittinger G, Lacarelle B, Antimicrob Agents Chemother 2003 47 238 243 10.1128/AAC.47.1.238-243.2003 12499197 (Pubitemid 36070370)
Antiretroviral drugs. De Clercq E, Curr Opin Pharmacol 2010 10 507 515
Novel antiretroviral combinations in treatmentexperienced patients with HIV infection: rationale and results. Taiwo B, Murphy RL, Katlama C, Drugs 70 1629 1642
Recent status of HIV-1 gene expression inhibitors. Baba M, Antiviral Res 2006 71 301 306 10.1016/j.antiviral.2006.01.002 16488488 (Pubitemid 44223751)
Transcription of HIV: Tat and cellular chromatin. Gatignol A, Adv Pharmacol 2007 55 137 159 17586314
The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention. Stevens M, De Clercq E, Balzarini J, Med Res Rev 2006 26 595 625 10.1002/med.20081 16838299 (Pubitemid 44230414)
Cellular factors as alternative targets for inhibition of HIV-1. Baba M, Antiviral Res 1997 33 141 152 10.1016/S0166-3542(96)01010-8 9037371 (Pubitemid 27060362)
New anti-HIV agents and targets. De Clercq E, Med Res Rev 2002 22 531 565 10.1002/med.10021 12369088 (Pubitemid 35299533)
Human immunodeficiency virus gene regulation as a target for antiviral chemotherapy. Daelemans D, Vandamme AM, De Clercq E, Antivir Chem Chemother 1999 10 1 14 10079874 (Pubitemid 29110119)
p27(SJ), a novel protein in St John's Wort, that suppresses expression of HIV-1 genome. Darbinian-Sarkissian N, Darbinyan A, Otte J, Radhakrishnan S, Sawaya BE, Arzumanyan A, Chipitsyna G, Popov Y, Rappaport J, Amini S, Khalili K, Gene Ther 2006 13 288 295 10.1038/sj.gt.3302649 16251997 (Pubitemid 43223097)
Identification of X-DING-CD4, a new member of human DING protein family that is secreted by HIV-1 resistant CD4(+) T cells and has anti-viral activity. Lesner A, Shilpi R, Ivanova A, Gawinowicz MA, Lesniak J, Nikolov D, Simm M, Biochem Biophys Res Commun 2009 389 284 289 10.1016/j.bbrc.2009.08.140 19720052
For whom the bell tolls? DING proteins in health and disease. Berna A, Bernier F, Chabriere E, Elias M, Scott K, Suh A, Cell Mol Life Sci 2009 66 2205 2218 10.1007/s00018-009-0006-6 19290474
Structural insights and ab initio sequencing within the DING proteins family. Elias M, Liebschner D, Gotthard G, Chabriere E, J Synchrotron Radiat 2011 18 45 49
DING proteins; novel members of a prokaryotic phosphate-binding protein superfamily which extends into the eukaryotic kingdom. Berna A, Bernier F, Chabriere E, Perera T, Scott K, Int J Biochem Cell Biol 2008 40 170 175 10.1016/j.biocel.2007.02.004 17368078 (Pubitemid 350137871)
Eukaryotic DING proteins are endogenous: an immunohistological study in mouse tissues. Collombet JM, Elias M, Gotthard G, Four E, Renault F, Joffre A, Baubichon D, Rochu D, Chabriere E, PLoS One 2010 5 9099
Crystallization and preliminary X-ray diffraction analysis of human phosphate-binding protein. Contreras-Martel C, Carpentier P, Morales R, Renault F, Chesne-Seck ML, Rochu D, Masson P, Fontecilla-Camps JC, Chabriere E, Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 62 67 69 16511265 (Pubitemid 43786811)
Serendipitous discovery and X-ray structure of a human phosphate binding apolipoprotein. Morales R, Berna A, Carpentier P, Contreras-Martel C, Renault F, Nicodeme M, Chesne-Seck ML, Bernier F, Dupuy J, Schaeffer C, et al. Structure 2006 14 601 609 10.1016/j.str.2005.12.012 16531243 (Pubitemid 43363492)
Tandem purification of two HDL-associated partner proteins in human plasma, paraoxonase (PON1) and phosphate binding protein (HPBP) using hydroxyapatite chromatography. Renault F, Chabriere E, Andrieu JP, Dublet B, Masson P, Rochu D, J Chromatogr B Analyt Technol Biomed Life Sci 2006 836 15 21 10.1016/j.jchromb.2006.03.029 16595195
Direct phasing at low resolution of a protein copurified with human paraoxonase (PON1). Fokine A, Morales R, Contreras-Martel C, Carpentier P, Renault F, Rochu D, Chabriere E, Acta Crystallogr D Biol Crystallogr 2003 59 2083 2087 10.1107/S0907444903017682 14646065 (Pubitemid 38024842)
Elucidation of the phosphate binding mode of DING proteins revealed by subangstrom Xray crystallography. Liebschner D, Elias M, Moniot S, Fournier B, Scott K, Jelsch C, Guillot B, Lecomte C, Chabriere E, J Am Chem Soc 2009 131 7879 7886 10.1021/ja901900y 19445459
[Discovery and crystallographic structure of human apolipoprotein]. Morales R, Berna A, Carpentier P, Contreras-Martel C, Renault F, Nicodeme M, Chesne-Seck ML, Bernier F, Dupuy J, Schaeffer C, et al. Ann Pharm Fr 2007 65 98 107 17404543 (Pubitemid 46457338)
A tale of the unexpected. Webb MR, Structure 2006 14 391 392 10.1016/j.str.2006.02.002 16531223 (Pubitemid 43363472)
Tandem use of X-ray crystallography and mass spectrometry to obtain ab initio the complete and exact amino acids sequence of HPBP, a human 38-kDa apolipoprotein. Diemer H, Elias M, Renault F, Rochu D, Contreras-Martel C, Schaeffer C, Van Dorsselaer A, Chabriere E, Proteins 2008 71 1708 1720 18076037 (Pubitemid 351732929)
Reconstitution of catalytically competent human zeta-thrombin by combination of zeta-thrombin residues A1-36 and B1-148 and an Escherichia coli expressed polypeptide corresponding to zeta-thrombin residues B149-259. Gan ZR, Lewis SD, Stone JR, Shafer JA, Biochemistry 1991 30 11694 11699 10.1021/bi00114a013 1751488
Characterization of human monocyte-derived microglia-like cells. Leone C, Le Pavec G, Meme W, Porcheray F, Samah B, Dormont D, Gras G, Glia 2006 54 183 192 10.1002/glia.20372 16807899 (Pubitemid 44230855)
Recruitment of Tat to heterochromatin protein HP1 via interaction with CTIP2 inhibits human immunodeficiency virus type 1 replication in microglial cells. Rohr O, Lecestre D, Chasserot-Golaz S, Marban C, Avram D, Aunis D, Leid M, Schaeffer E, J Virol 2003 77 5415 5427 10.1128/JVI.77.9.5415-5427.2003 12692243 (Pubitemid 36460955)
Apoptosis of CD8+ T cells is mediated by macrophages through interaction of HIV gp120 with chemokine receptor CXCR4. Herbein G, Mahlknecht U, Batliwalla F, Gregersen P, Pappas T, Butler J, O'Brien WA, Verdin E, Nature 1998 395 189 194 10.1038/26026 9744279 (Pubitemid 28425660)
HIV with reduced sensitivity to zidovudine (AZT) isolated during prolonged therapy. Larder BA, Darby G, Richman DD, Science 1989 243 1731 1734 10.1126/science.2467383 2467383 (Pubitemid 19106971)
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Mosmann T, J Immunol Methods 1983 65 55 63 10.1016/0022-1759(83)90303-4 6606682 (Pubitemid 14203433)
Evidence for phosphatase activity of p27SJ and its impact on the cell cycle. Darbinian N, Czernik M, Darbinyan A, Elias M, Chabriere E, Bonasu S, Khalili K, Amini S, J Cell Biochem 2009 107 400 407 10.1002/jcb.22135 19343785
Suppression of HIV-1 transcriptional elongation by a DING phosphatase. Darbinian N, Gomberg R, Mullen L, Garcia S, White MK, Khalili K, Amini S, J Cell Biochem 2011 112 225 232
St. John's Wort protein, p27SJ, regulates the MCP-1 promoter. Mukerjee R, Deshmane SL, Darbinian N, Czernik M, Khalili K, Amini S, Sawaya BE, Mol Immunol 2008 45 4028 4035 10.1016/j.molimm.2008.06.001 18649942
Tenofovir exhibits low cytotoxicity in various human cell types: comparison with other nucleoside reverse transcriptase inhibitors. Cihlar T, Birkus G, Greenwalt DE, Hitchcock MJ, Antiviral Res 2002 54 37 45 10.1016/S0166-3542(01)00210-8 11888656 (Pubitemid 34219173)
Relative anti-HIV-1 efficacy of lamivudine and emtricitabine in vitro is dependent on cell type. Hazen R, Lanier ER, J Acquir Immune Defic Syndr 2003 32 255 258 10.1097/00126334-200303010-00003 12626884 (Pubitemid 36331696)
Human immunodeficiency virus type 1 LTR TATA and TAR region sequences required for transcriptional regulation. Garcia JA, Harrich D, Soultanakis E, Wu F, Mitsuyasu R, Gaynor RB, Embo J 1989 8 765 778 2721501 (Pubitemid 19273248)
The regulation of human immunodeficiency virus type-1 gene expression. Kingsman SM, Kingsman AJ, Eur J Biochem 1996 240 491 507 10.1111/j.1432-1033. 1996.0491h.x 8856047 (Pubitemid 26321148)
A compilation of cellular transcription factor interactions with the HIV-1 LTR promoter. Pereira LA, Bentley K, Peeters A, Churchill MJ, Deacon NJ, Nucleic Acids Res 2000 28 663 668 10.1093/nar/28.3.663 10637316 (Pubitemid 30068248)
Regulation of HIV-1 gene transcription: from lymphocytes to microglial cells. Rohr O, Marban C, Aunis D, Schaeffer E, J Leukoc Biol 2003 74 736 749 10.1189/jlb.0403180 12960235 (Pubitemid 38040350)
Treatment of antiretroviral-drug-resistant HIV-1 infection. Deeks SG, Lancet 2003 362 2002 2011 10.1016/S0140-6736(03)15022-2 14683662 (Pubitemid 37548427)
Drug-resistant human immunodefiency virus. Kozal MJ, Clin Microbiol Infect 2009 15 Suppl 1 69 73 19220361
Stabilization of the active form(s) of human paraoxonase by human phosphate-binding protein. Rochu D, Chabriere E, Renault F, Elias M, Clery-Barraud C, Masson P, Biochem Soc Trans 2007 35 1616 1620 10.1042/BST0351616 18031277
Serum paraoxonase-1 activity and concentration are influenced by human immunodeficiency virus infection. Parra S, Alonso-Villaverde C, Coll B, Ferre N, Marsillach J, Aragones G, Mackness M, Mackness B, Masana L, Joven J, Camps J, Atherosclerosis 2007 194 175 181 10.1016/j.atherosclerosis.2006.07.024 16942773 (Pubitemid 47464297)
Paraoxonase-1 gene haplotypes are associated with metabolic disturbances, atherosclerosis, and immunologic outcome in HIV-infected patients. Parra S, Marsillach J, Aragones G, Beltran R, Montero M, Coll B, Mackness B, Mackness M, Alonso-Villaverde C, Joven J, Camps J, J Infect Dis 2010 201 627 634