Gynogenesis induction and sex determination in the Eurasian perch, Perca fluviatilis

[en] In the present study, we used meiotic gynogenesis, widely used in studies on sex determination, to confirm female homogamety in Eurasian perch, Perca fluviatilis. Sperm irradiated with UV for 400 s was used to artificially fertilized eggs. The diploid of the resulting embryos was restored by a heat shock (30 degreesC) applied to the eggs 5 min postfertilization, for 25 min. Fertilization (ranging between 45% and 75%) and survival rates at hatching (ranging between 3.4% and 46.6%) were not significantly different (P>0.05) between the diploid control and gynogenetics. The diploid controls and two batches of gynogenetics contained 100% diploid larvae, whereas two other batches of gynogenetics contained 6.7% and 10.0% triploid larvae. The sex ratios of the diploid controls were not significantly different from 1:1, whereas all gynogenetic families were 100% female. These results confirm female homogamety in Eurasian perch, demonstrated by the use of hormonally mascilinized breeders in a previous study. (C) 2004 Elsevier B.V. All rights reserved.

Disciplines :

Aquatic sciences & oceanology

Author, co-author :

Rougeot, Carole ; Université de Liège - ULiège > Centre de formation et de recherche en aquaculture (CEFRA)

Ngingo, J. V.

Gillet, Laurent ; Université de Liège - ULiège > Immunologie et vaccinologie

Vanderplasschen, Alain ; Université de Liège - ULiège > Immunologie et vaccinologie

Mélard, Charles ; Université de Liège - ULiège > Centre de formation et de recherche en aquaculture (CEFRA)

Language :

English

Title :

Gynogenesis induction and sex determination in the Eurasian perch, Perca fluviatilis

Publication date :

03 January 2005

Journal title :

Aquaculture

ISSN :

0044-8486

eISSN :

1873-5622

Publisher :

Elsevier Science Bv, Amsterdam, Netherlands

Volume :

243

Issue :

1-4

Pages :

411-415

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 15 June 2009

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