[en] Expression of the SRIH gene was investigated in six human normal anterior pituitaries, six GH-, three PRL-, three mixed GH/PRL-secreting and four nonsecreting adenomas. Total cellular RNA and poly(A+) mRNAs were analyzed by dot and Northern blot hybridization to a 3'-end labeled oligonucleotide probe specific for the human pre-proSRIH mRNA. A weak but detectable pre-proSRIH hybridization signal was present in human normal anterior pituitaries and in the four groups of adenomas. The size of this pre-proSRIH mRNA was indistinguishable from that found in our hypothalamic samples and close to that described in the literature. The wide variation of the signal intensity from one case to the other in each group of the different types of normal and tumoral antehypophyseal samples prevented establishment of any correlation between the level of pre-proSRIH mRNA and the nature of the pituitary tissue. The presence of SRIH mRNA in human normal and tumoral anterior pituitary tissues provides a sound basis to substantiate the hypothesis of a SRIH biosynthesis in the human anterior pituitary gland.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Pagesy, P.
Li, J. Y.
Rentier-Delrue, Françoise ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique
Le Bouc, Y.
Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Peillon, F.
Language :
English
Title :
Evidence of pre-prosomatostatin mRNA in human normal and tumoral anterior pituitary gland
Publication date :
1989
Journal title :
Molecular Endocrinology
ISSN :
0888-8809
eISSN :
1944-9917
Publisher :
Endocrine Society, Chevy Chase, United States - Maryland
Childs GV, Cole DE, Kubek M, Tobin R, Wilber JF, 1978 Endogenous thyrotropin releasing hormone in the anterior pituitary: sites of activity as identified immunocytochemi-cal staining. J Histochem Cytochem 24:901-908
Khachaturian H, Sherman TG, Lloyd RV, Civelli O, Douglas J, Nerbert ED, Akil H, Watson SJ, 1986 Pro-dynorphin is endogenous to the anterior pituitary and is colocalized with LH and FSH in the gonadotrophs. Endocrinology 119:1409-1411
Rehfeld JF 1987 Preprocholecystokinin processing in the normal human anterior pituitary. Proc Natl Acad Sci USA 84:3019-3023
Mesguich P, Benoit R, Dubois PM, Morel G, 1988 Somatostatin-28- and somatostatin-14-like immunoreactivities in the rat pituitary gland. Cell Tissue Res 252:419-427
Wilber JF, Montoya E, Plotnikoff ND, 1976 Gonadotropin releasing hormone and thyrotropin releasing hormone: distribution and effects on central nervous system. Recent Prog Horm Res 32:117-159
Li JY, Knapp RJ, Sternberger LA, 1984 Immunocytochem-istry of a “private” luteinizing-releasing hormone system in the pituitary. Cell Tissue Res 253:263-266
May V, Wilber JF, U’Prichard DC, Childs GV, 1987 Persistence of immunoreactive TRH and GnRH in long-term primary anterior pituitary cultures. Peptides 8:543-558
Arnaout MA, Garthwaite TL, Martison DA, Hagen TC, 1986 Vasoactive intestinal peptide is synthesized in anterior pituitary tissue. Endocrinology 119:2052-2057
Denef C, Baes M, Schramme C, 1986 Paracrine interactions in the anterior pituitary: role in the regulation of prolactin and growth hormone secretion. In: Ganong W, Martini L (eds) Frontiers in Neuroendocrinology. Raven Press, New York, pp 115-148
Nagy G, Mulchahey JF, Neill JD, 1988 Autocrine control of prolactin secretion by vasoactive intestinal peptide. Endocrinology 122:364-366
Le Dafniet M, Grouselle D, Li JY, Kujas M, Bression D, Barret A, Tixier-Vidal A, Peillon F, 1987 Evidence of thyrotropin releasing hormone (TRH) and TRH binding sites in human nonsecreting pituitary adenomas. J Clin Endocrinol Metab 65:1014-1019
Le Dafniet M, Blumberg-Tick J, Li JY, Brandi AM, Bression D, Barret A, Feinstein MC, Peillon F, 1988 Liberation de thyrolibérine (TRH) par les cellules adénomateuses hypophysaires humaines à prolactine. Modulation par la dopamine. C R Acad Sci Paris 306:129-133
Joubert D, Benlot C, Lagoguey A, Garnier P, Brandi AM, Gautron JP, Legrand JC, Peillon F, 1989 Normal and growth hormone (GH)-secreting adenomatous human pituitaries release somatostatin and GH-re-leasing hormone. J Clin Endocrinol Metab 68:572-577
Peillon F, Liappi G, Gamier P, Brandi AM, Evain-Brion D, Dodeur M, Gautron JP, Donnadieu M, Michard M, Joubert D, 1988 Sécrétion in vitro de somatostatine (SR I H) par les cellules somatotropes adénomateuses humaines. Relation avec la libération d’hormone somatotrope (GH) et modulation par la thyrolibérine (TRH). C R Acad Sci Paris 306:161-165
Peillon F, Le Dafniet M, Garnier Ph, Brandi AM, Moyse E, Birman P, Blumberg-Tick J, Grouselle D, Joubert D, 1989 Receptors and neurohormones studies in human pituitary adenomas. Hormone Res 31:13-18
Shen LP, Pictet RL, Rutter WJ, 1982 Human somatostatin I: sequence of the cDNA. Proc Natl Acad Sci USA 79:4575-4579
Martial JA, Hallewell RA, Baxter JD, Goodman HM, 1979 Human growth hormone: complementary DNA cloning and expression in bacteria. Science 205:602-607
Berry SA, Pescovitz OH, 1988 Identification of a rat GHRH-like substance and its messenger RNA in rat testis. Endocrinology 123:661-663
Scanlon MF, Robbins AJ, Bulaffi JL, Jackson IMD, Reichlin S, 1983 Characterization of somatostatin and TRH release by rat hypothalamic and cerebral cortical neurons maintained on a capillary membrane perifusion system. Neuroendocrinology 37:269-274
Robbins RJ, Sutton RE, Reichlin S, 1982 Sodium and calcium dependent somatostatin release from dissociated cerebral cortical cells in culture. Endocrinology 110:496-499
Uhl GR, Zingg HH, Habener JF, 1985 Vasopressin mRNA “in situ” hybridization: localization and regulation studied with oligonucleotide cDNA probes in normal Brattelboro rat hypothalamus. Proc Natl Acad Sci USA 83:5555-5559
Fuller PJ, Clements JA, Funder JW, 1985 Localization of arginine vasopressin-neurophysin II messenger ribonucleic acid in the hypothalamus of control and Brattelboro rats by hybridization histochemistry with a synthetic pen-tadecamer oligonucleotide probe. Endocrinology 116:2366-2368
Bloch B, Popovici T, Chouam S, Kosalski C, 1986 Detection of the mRNA coding for enkephalin precursor in rat brain and adrenal by using an “in situ” hybridization procedure. Neurosci Lett 64:29-34
Low MJ, Hammer RE, Goodman RH, Habener JF, Palmiter RD, Brinster RL, 1986 Tissue-specific posttranslational processing of pre-prosomatostatin encoded by a metal-lothionein-somatostatin fusion gene in transgenic mice. Cell 41:211-219
Moyse E, Le Dafniet M, Epelbaum J, Pagesy P, Peillon F, Kordon C, Enjalbert A, 1985 Somatostatin receptors in human growth hormone and prolactin-secreting pituitary adenomas. J Clin Endocrinol Metab 61:98-103
Reubi JC, Heitz PU, Landolt AM, 1987 Visualization of somatostatin receptors and correlation with immunoreactive growth hormone and prolactin in human pituitary adenomas: evidence for different tumor subclasses. J Clin Endocrinol Metab 65:65-73
Herlant M, 1960 Etude critique de deux techniques nouvelles destinées à mettre en évidence les différentes catégories cellulaires présentes dans la glande pituitaire. Bull Microsc Appl 10:37-44
Mazzuca M, Dubois MP, 1974 Detection of luteinizing hormone releasing hormone in the guinea pig median eminence with an immunohistoenzymatic technique. J Histochem Cytochem 22:993-996
Roychoudhury R, Wu R, 1980 Terminal transferase-catalyzed addition of nucleotides to the 3′termini of DNA. Methods Enzymol 65:43-62
Chirgwin JM, Przybyla AE, MacDonald PJ, Rutter WJ, 1979 Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294-5299
Thomas PS, 1980 Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77:5201-5205