Poster (Scientific congresses and symposiums)
Development of nanoprobes for cancerous tissues imaging by Surface-Enhanced Raman Spectroscopy
Müller, Wendy; Verdin, Alexandre; Malherbe, Cédric et al.
2018Bioforum 2018
 

Files


Full Text
Poster BioForum Wendy.pdf
Author postprint (4.23 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Raman; SERS; imaging; nanoprobe; cancerous tissues
Abstract :
[en] Early detection and precise characterization of cancers is one of the major challenges in medicine. Consequently, there is a strong need to develop highly sensitive, specific and quick analytical techniques. Among the emerging methods allowing the analysis of cancerous samples, Surface-Enhanced Raman Spectroscopy (SERS), an amplified technique of vibrational spectroscopy based on Raman scattering, is particularly suitable. The signal enhancement in SERS, up to 10^12-fold in the presence of metallic nanoparticles near the Raman-active molecule, offers a better sensitivity than in “classic” Raman spectroscopy and thus allows the analysis of molecules in weaker concentrations. One of the advantages of the nanoparticles used in SERS is that they can be functionalized in order to specifically target a molecule of interest and therefore give information about the spatial location of biomarkers via SERS imaging. Our research aims at developing SERS nanoprobes functionalized with folic acid, complementary to the FRalpha receptor (folate receptor alpha), a membrane receptor overexpressed in many types of cancers. SERS imaging with these nanoprobes will then enable to spot cancerous areas within human cancerous tissues and to discriminate between healthy and cancerous tissues. The preliminary results of our study show that nanoprobes can bind to tissues and more particularly at the level of membrane structures and that they can be detected via SERS imaging. SERS imaging also allows to observe a signal accumulation in presumed cancerous areas, in correlation with the histological H&E analysis. Finally, SERS imaging of healthy and cancerous tissues seems to indicate higher signal intensity within cancerous tissues than within healthy tissues.
Research center :
Laboratoire de Chimie Analytique Inorganique
Disciplines :
Chemistry
Author, co-author :
Müller, Wendy  ;  Université de Liège - ULiège > Master en sc. chimiques, à fin.
Verdin, Alexandre  ;  Université de Liège - ULiège > Département de chimie (sciences) > Département de chimie (sciences)
Malherbe, Cédric ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Eppe, Gauthier  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Language :
English
Title :
Development of nanoprobes for cancerous tissues imaging by Surface-Enhanced Raman Spectroscopy
Publication date :
17 May 2018
Event name :
Bioforum 2018
Event organizer :
Bioliège
Event place :
Liège, Belgium
Event date :
17 Mai 2018
Audience :
International
Available on ORBi :
since 22 May 2018

Statistics


Number of views
232 (60 by ULiège)
Number of downloads
8 (8 by ULiège)

Bibliography


Similar publications



Contact ORBi