Article (Scientific journals)
Exploring with [(18)F]UCB-H the in vivo Variations in SV2A Expression through the Kainic Acid Rat Model of Temporal Lobe Epilepsy.
Serrano Navacerrada, Maria Elisa; Bahri, Mohamed Ali; Becker, Guillaume et al.
2020In Molecular Imaging and Biology
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Keywords :
EEG; Kainic acid; SV2A; SV2A immunofluorescence; T2 MRI; Temporal lobe epilepsy; [18F]UCB-H
Abstract :
[en] PURPOSE: The main purpose of this study was to understand how the positron emission tomography (PET) measure of the synaptic vesicle 2A (SV2A) protein varies in vivo during the development of temporal lobe epilepsy (TLE) in the kainic acid rat model. PROCEDURES: Twenty Sprague Dawley male rats were administered with multiple systemic doses of saline (control group, n = 5) or kainic acid (5 mg/kg/injection, epileptic group, n = 15). Both groups were scanned at the four phases of TLE (early, latent, transition, and chronic phase) with the [(18)F]UCB-H PET radiotracer and T2-structural magnetic resonance imaging. At the end of the scans (3 months post-status epilepticus), rats were monitored for 7 days with electroencephalography for the detection of spontaneous electrographic seizures. Finally, the immunofluorescence staining for SV2A expression was performed. RESULTS: Control rats presented a significant increase in [(18)F]UCB-H binding at the last two scans, compared with the first ones (p < 0.001). This increase existed but was lower in epileptic animals, producing significant group differences in all the phases of the disease (p < 0.028). Furthermore, the quantification of the SV2A expression in vivo with the [(18)F]UCB-H radiotracer or ex vivo with immunofluorescence led to equivalent results, with a positive correlation between both. CONCLUSIONS: Even if further studies in humans are required, the ability to detect a progressive decrease in SV2A expression during the development of temporal lobe epilepsy supports the use of [(18)F]UCB-H as a useful tool to differentiate, in vivo, between healthy and epileptic animals along with the development of the epileptic disease.
Research center :
GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging & Memory - ULiège
Disciplines :
Radiology, nuclear medicine & imaging
Author, co-author :
Serrano Navacerrada, Maria Elisa ;  Université de Liège - ULiège > CRC In vivo Imaging-Preclinical Imaging
Bahri, Mohamed Ali  ;  Université de Liège - ULiège > CRC In vivo Imaging-Aging & Memory
Becker, Guillaume ;  Université de Liège - ULiège > Département de Psychologie > Département de Psychologie
Seret, Alain ;  Université de Liège - ULiège > Département de physique > Imagerie médicale expérimentale
Germonpre, Charlotte
Lemaire, Christian ;  Université de Liège - ULiège > CRC In vivo Imaging-Radiochemistry
Giacomelli, Fabrice 
Mievis, Frédéric 
Luxen, André ;  Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de chimie organique de synthèse
Salmon, Eric  ;  Université de Liège - ULiège > Département des sciences cliniques > Neuroimagerie des troubles de la mémoire et revalid. cogn.
Rogister, Bernard  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Raedt, Robrecht
Plenevaux, Alain  ;  Université de Liège - ULiège > CRC In vivo Imaging-Preclinical Imaging
More authors (3 more) Less
Language :
English
Title :
Exploring with [(18)F]UCB-H the in vivo Variations in SV2A Expression through the Kainic Acid Rat Model of Temporal Lobe Epilepsy.
Publication date :
23 March 2020
Journal title :
Molecular Imaging and Biology
ISSN :
1536-1632
eISSN :
1860-2002
Publisher :
Springer, United States - New York
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
ULiège - Université de Liège [BE]
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