Infectious Agents Identified by Real-Time PCR, Serology and Bacteriology in Blood and Peritoneal Exudate Samples of Cows affected by Parietal Fibrinous Peritonitis after Caesarean Section
[en] The aim of this study was to identify the pathogens potentially involved in parietal fibrinous peritonitis (PFP). PFP is a complication of laparotomy in cattle, characterized by an accumulation of exudate inside a fibrinous capsule. We have studied 72 cases of PFP in Belgian blue cows, confirmed by a standard diagnostic protocol. Blood was collected to evaluate the presence of antibodies for Mycoplasma bovis (M. bovis), Coxiella burnetii (C. burnetii) and Bovine Herpesvirus 4 (BoHV4) by enzyme-linked immunosorbent assays. Peritoneal exudate was obtained from the PFP cavity to perform bacteriological culture, and to identify the DNA of M. bovis, C. burnetii and BoHV4 using real time polymerase chain reaction (qPCR). Bacteriological culture was positive in most peritoneal samples (59/72); Trueperella pyogenes (T. pyogenes) (51/72) and Escherichia coli (E. coli) (20/72) were the most frequently identified. For BoHV4, the majority of cows showed positive serology and qPCR (56/72 and 49/72, respectively). Contrariwise, M. bovis (17/72 and 6/72, respectively) and C. burnetii (15/72 and 6/72, respectively) were less frequently detected (p < 0.0001). Our study proves that PFP can no longer be qualified as a sterile inflammation. Moreover, we herein describe the first identification of BoHV4 and C. burnetii in cows a ected by PFP.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Djebala, Salem ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Médecine interne des ruminants et des porcs
Evrard, Julien; Regional Association of Health and Animal Identification > Gestion et Prévention de Santé > Gestion et Prévention de Santé > DMV
Gregoire, Fabien; Regional Association of Health and Animal Identification, > Gestion et Prévention de Santé > Gestion et Prévention de Santé > DMV
Thiry, Damien ; University of Liege > Department of Infectious and Parasitic Diseases > Bacteriology > DMV, MVPH, PHD, Dipl ECVM
Bayrou, Calixte ; University of Liège > Clinical Department of Ruminants > Clinical Department of Ruminants > DMV, PHD, ECBHM resisdent
Moula, Nassim ; University of Liege > Department of Animal Production > Department of Animal Production > DMV, PHD
Sartelet, Arnaud ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Département clinique des animaux de production (DCP)
Bossaert, Philippe ; Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Thériogenologie des animaux de production
Language :
English
Title :
Infectious Agents Identified by Real-Time PCR, Serology and Bacteriology in Blood and Peritoneal Exudate Samples of Cows affected by Parietal Fibrinous Peritonitis after Caesarean Section
Braun, U.; Pusterla, N.; Anliker, H. Ultrasonographic findings in three cows with peritonitis in the left flank region. Vet. Rec. 1998, 142, 338-340.
Gille, L.; Pilo, P.; Valgaeren, B.R.; van Driessche, L.; van Loo, H.; Bodmer, M.; Burki, S.; Boyen, F.; Haesebrouck, F.; Deprez, P.; et al. A new predilection site of Mycoplasma bovis: Postsurgical seromas in beef cattle. Vet. Microbiol. 2016, 186, 67-70.
Djebala, S.; Evrard, J.; Moula, N.; Gille, L.; Bayrou, C.; Eppe, J.; Casalta, H.; Sartelet, A.; Bossaert, P. Comparison between generalised peritonitis and parietal fibrinous peritonitis in cows after caesarean section. Vet. Rec. 2020, 105867.
Djebala, S.; Evrard, J.; Moula, N.; Sartelet, A.; Bossaert, P. Atypical case of parietal fibrinous peritonitis in a Belgian Blue heifer without a history of laparotomy. Vet. Rec. Case Rep. 2020, 8, e001086.
Bourdette, P.; Mirbach, S.; Hoolbecq, M.; Plassard, V.; El-Bay, S.; Millemann, Y. "Clapier collé": Un cas original de péritonite chez une montbéliarde en France. Point. Vét. 2017, 372, 20-23.
Djebala, S.; Moula, N.; Bayrou, C.; Sartelet, A.; Bossaert, P.; Calixte, B.; Arnaud, S.; Philippe, B. Prophylactic antibiotic usage by Belgian veterinarians during elective caesarean section in Belgian blue cattle. Prev. Vet. Med. 2019, 172, 104785.
Mijten, P. Puerperal Complications After Cesarean Section in Dairy Cows and in Double-Muscled Cows. Reprod. Domest. Anim. 1998, 33, 175-179.
Lamain, G.; Touati, K.; Rollin, F. La peritonite fibrineuse parietale: Une complication dans l'espèce bovine. In Proceedings of the Groupement Technique Vétérinaire Conference, Nante, France, 26-28 May 2012; pp. 237-240.
Lamain, G.; Touati, K.; Rollin, F. Aseptic fibrinous parietal peritonitis in cattle: Retrospective and clinical study. In Proceedings of the European Buiatrics Forum Conference, Marseille, France, 1-3 December 2009; p. 115.
Donofrio, G.; Flammini, C.F.; Scatozza, F.; Cavirani, S. Detection of Bovine Herpesvirus 4 (BoHV-4) DNA in the Cell Fraction of Milk of Dairy Cattle with History of BoHV-4 Infection. J. Clin. Microbiol. 2000, 38, 4668-4671.
Bellino, C.; Iussich, S.; Biasato, I.; Peletto, S.; Caruso, C.; Gianella, P.; Cagnasso, A.; D'Angelo, A. Potential Pathogenetic Role of Bovine Herpesvirus 4 in Two Dairy Cows with Dermatitis-Pyrexia-Hemorrhagic Syndrome. J. Clin. Microbiol. 2015, 53, 2763-2767.
Szymańska-Czerwińska, M.; Niemczuk, K.; Jodelko, A. Evaluation of qPCR and phase I and II antibodies for detection of Coxiella burnetii infection in cattle. Res. Vet. Sci. 2016, 108, 68-70.
Association Régionale de Santé et d'Identification Animales (Annual Report). Available online: https: //www.arsia.be/wp-content/uploads/documents-telechargeables/RA-2017-light-Quality.pdf (accessed on 25 May 2020).
Delooz, L.; Czaplicki, G.; Houtain, J.Y.; Pozzo, F.D.; Saegerman, C. Laboratory Findings Suggesting an Association Between BoHV-4 and Bovine Abortions in Southern Belgium. Transbound. Emerg. Dis. 2016, 64, 1100-1109.
Wellemans, G.; Antoine, H.; Broes, A.; Charlier, G.; van Opdenbosch, E. Symptomatologie variée apparaissant lors de métrites chroniques associées au virus herpès chez les bovins. Ann. Méd. Vét. 1984, 128, 65-74.
Koskinen, M.; Wellenberg, G.; Sampimon, O.; Holopainen, J.; Rothkamp, A.; Salmikivi, L.; van Haeringen, W.; Lam, T.; Pyorala, S. Field comparison of real-time polymerase chain reaction and bacterial culture for identification of bovine mastitis bacteria. J. Dairy Sci. 2010, 93, 5707-5715.
Di Gioia, D.; Mazzola, G.; Nikodinoska, I.; Aloisio, I.; Langerholc, T.; Rossi, M.; Raimondi, S.; Melero, B.; Rovira, J. Lactic acid bacteria as protective cultures in fermented pork meat to prevent Clostridium spp. growth. Int. J. Food Microbiol. 2016, 235, 53-59.
Mijten, P.; van den Bogaard, A.E.J.M.; Hazen, M.J.; de Kruif, A. Bacterial contamination of fetal fluids at the time of caesarean section in the cow. Theriogenology 1996, 97, 513-521.
Mohammed, A.N. Field study on evaluation of the efficacy and usability of two disinfectants for drinking water treatment at small cattle breeders and dairy cattle farms. Environ. Monit. Assess. 2016, 188, 151-162.
Rzewuska, M.; Kwiecień, E.; Chrobak-Chmiel, D.; Kizerwetter-Swida, M.; Śtefańska, I.; Gieryńska, M. Pathogenicity and Virulence of Trueperella pyogenes: A Review. Int. J. Mol. Sci. 2019, 20, 2737.
Credille, B.; Woolums, A.; Giguère, S.; Robertson, T.; Overton, M.; Hurley, D. Prevalence of Bacteremia in Dairy Cattle with Acute Puerperal Metritis. J. Vet. Intern. Med. 2014, 28, 1606-1612.
Kolenda, R.; Burdukiewicz, M.; Schierack, P. A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli. Front. Microbiol. 2015, 5, 1-12.
Fecteau, G. Management of peritonitis in cattle. Vet. Clin. N. Am. Food Anim. Pract. 2005, 21, 155-171.
Jones, S.I.; Smith, B.P. Disease of the alimentary tract. In Large Animal Internal Medicine, 5th ed.; Smith, B.P., Ed.; Elsevier: St. Louis, MO, USA, 2015; pp. 638-842.
Ducharme, N.G.; des Rochers, A.; Fubini, S.L.; Pease, A.P.; Mizer, L.A.; Walker, W.; Trent, A.M.; Roy, J.-P.; Rousseau, M.; Radcliffe, R.M.; et al. Surgery of the Bovine Digestive System. In Farm Animal Surgery; Elsevier BV: Amsterdam, The Netherlands, 2017; pp. 223-343.
Sheldon, I.M.; Williams, E.J.; Miller, A.N.; Nash, D.; Herath, S. Uterine diseases in cattle after parturition. Vet. J. 2008, 176, 115-121.
Tamai, I.A.; Mohammadzadeh, A.; Salehi, T.Z.; Mahmoodi, P. Genomic characterisation, detection of genes encoding virulence factors and evaluation of antibiotic resistance of Trueperella pyogenes isolated from cattle with clinical metritis. Antonie Leeuwenhoek 2018, 111, 2441-2453.
Engels, M.; Ackermann, M. Pathogenesis of ruminant herpesvirus infections. Vet. Microbiol. 1996, 53, 3-15.
Raoult, D.; Marrie, T.; Mege, J. Natural history and pathophysiology of Q fever. Lancet Infect. Dis. 2005, 5, 219-226.
Maunsell, F.P.; Woolums, A.; Francoz, D.; Rosenbusch, R.; Step, D.; Wilson, D.; Janzen, E. Mycoplasma bovis Infections in Cattle. J. Vet. Intern. Med. 2011, 25, 772-783.
Yang, B.; Li, Y.; Pascottini, O.; Xie, J.; Wei, R.; Opsomer, G.; Nauwynck, H. Primary replication and invasion of the bovine gammaherpesvirus BoHV-4 in the genital mucosae. Vet. Res. 2017, 48, 83.
Zhang, R.; Han, X.; Chen, Y.; Mustafa, R.; Qi, J.; Chen, X.; Hu, C.; Chen, H.; Guo, A. Attenuated Mycoplasma bovis strains provide protection against virulent infection in calves. Vaccine 2014, 32, 3107-3114.
Czaplicki, G.; Houtain, J.Y.; Mullender, C.; Porter, S.R.; Humblet, M.F.; Manteca, C.; Saegerman, C. Apparent prevalence of antibodies to Coxiella burnetii (Q fever) in bulk tank milk from dairy herds in southern Belgium. Vet. J. 2012, 192, 529-531.
Wellemans, G.; van Opdenbosch, E.; Mammerickx, M. Inoculation expérimentale du virus LVR 140 (HERPÈS BOVIN IV) à des vaches gestantes et non-gestantes. Ann. Rech. Vét. 1986, 17, 89-94.
Haapala, V.; Pohjanvirta, T.; Vähänikkilä, N.; Halkilahti, J.; Simonen, H.; Pelkonen, S.; Soveri, T.; Simojoki, H.; Autio, T. Semen as a source of Mycoplasma bovis mastitis in dairy herds. Vet. Microbiol. 2018, 216, 60-66.
Pardon, B.; de Bleecker, K.; Dewulf, J.; Callens, J.; Boyen, F.; Catry, B.; Deprez, P. Prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves. Vet. Rec. 2011, 169, 278.
Czaplicki, G.; Thiry, E. An association exists betweenbovine herpesvirus-4 seropositivity and abortion in cows. Prev. Med. Vet. 1998, 33, 233-240.
Klamminger, S.; Prunner, I.; Giuliodori, M.; Drillich, M. Uterine infection with bovine herpesvirus type 4 in dairy cows. Reprod. Domest. Anim. 2016, 52, 115-121.
Kreizinger, Z.; Szeredi, L.; Bacsadi, Á.; Nemes, C.; Sugár, L.; Varga, T.; Sulyok, K.M.; Szigeti, A.; Ács, K.; Tóbiás, E.; et al. Occurrence of Coxiella burnetii and Chlamydiales species in abortions of domestic ruminants and in wild ruminants in Hungary, Central Europe. J. Vet. Diagn. Investig. 2015, 27, 206-210.
Frazier, K.; Pence, M.; Mauel, M.J.; Liggett, A.; Hines, M.E.; Sangster, L.; Lehmkuhl, H.D.; Miller, D.; Styer, E.; West, J.; et al. Endometritis in postparturient cattle associ-ated with bovine herpesvirus-4 infection: 15 Cases. J. Vet. Diagn. Investig. 2001, 13, 502-508.
Donofrio, G.; Cavirani, S.; van Santen, V.L.; Flammini, C.F. Potential Secondary Pathogenic Role for Bovine Herpesvirus 4. J. Clin. Microbiol. 2005, 43, 3421-3426.
