Neospora caninum; direct ELISA; IFAT; C-ELISA; dog; seroprevalence
Abstract :
[en] An enzyme-linked immunosorbent assay (ELISA) was developed in our laboratory and used to determine the seroprevalence of Neospora caninum in three different dog populations in Belgium: healthy dogs from cattle farms and urban dogs with or without various neurological disorders. The test was validated and compared with two other tests: an indirect fluorescent antibody test (IFAT) and a commercially available competitive enzyme-linked immunosorbent assay (C-ELISA). The study showed a good correlation between the IFAT and the ELISA developed. When the two tests were compared with the C-ELISA, moderate positive and negative agreement indices were observed. Using our ELISA and the IFAT techniques, a high prevalence was found in farm dogs. This result showed that the neurological symptoms are not usually associated with the Neospora infection. In conclusion, the ELISA developed in our laboratory could replace the IFAT for the screening of a large number of dogs' sera. (C) 2004 Elsevier B.V. All rights reserved.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...) Veterinary medicine & animal health Entomology & pest control
Author, co-author :
Lasri, S.
De Meerschman, F.
Rettigner, C.
Focant, Charles ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Comparison of three techniques for the serological diagnosis of Neospora caninum in the dog and their use for epidemiological studies
Agresti, A., 1996. An Introduction to Categorical Data Analysis. Wiley, New York.
Anderson M.L., Blanchard P.C., Barr B.C., Dubey J.P., Hoffman R.L., Conrad P.A. Neospora-like protozoan infection as a major cause of abortion in California dairy cattle. J. Am. Med. Assoc. 198:1991;241-244
Baszler T.V., Adams S., Schalie J.V., Mathison B.A., Kostovic M. Validation of a commercially available monoclonal antibody-based competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to Neospora caninum in cattle. J. Clin. Microbiol. 39:2001;3851-3857
Björkman C., Uggla A. Serological diagnosis of Neospora caninum infection. Int. J. Parasitol. 29:1999;1497-1507
Björkman C., Lunden A., Holmadahl J., Barber J., Trees A.J., Uggla A. Neospora caninum in dogs: detection of antibodies by ELISA using an iscom antigen. Parasite Immunol. 16:1994;643-648
Cicchetti D.V., Feinstein A.R. High agreement but low kappa. II. Resolving the paradoxes. J. Clin. Epidemiol. 43:1990;551-558
De Meerschman, F., Czaplicki, G., Focant, C., Boreux, R., Leclipteux, T., Losson, B., 2000. Cattle neosporosis in Belgium: a case-control study in dairy and beef cattle. In: Hemphill, A., Gottstein, B. (Eds.), An European Perspective on Neospora caninum. Int. J. Parasitol. 30, 877-924.
Dubey J.P., Lindsay D.S. A review of Neospora caninum and neosporosis. Vet. Parasitol. 67:1996;1-59
Dubey J.P., Lindsay D.S. Gerbils (Meriones unguiculatus) are highly susceptible to oral infection with Neospora caninum oocysts. Parasitol. Res. 86:2000;165-168
Dubey J.P., Hattel A.L., Lindsay D.S., Topper M.J. Neonatal Neospora caninum infection in dogs: isolation of the causative agent and experimental transmission. J. Am. Vet. Med. Assoc. 193:1988;1259-1263
Dubey, J.P., Lindsay, D.S., Adams, D.S., et al., 1996. Serological responses of cattle and other animals infected with Neospora caninum. Am. J. Vet. Res., 329-336.
Dubey J.P., Jenkins M.C., Adams D.S., MacAllister M.M., Anderson-Sprecher R., Baszler T.V., Lally N.C., Björkman C., Uggla A. Antibody responses of cows during an outbreak of neosporosis evaluated by indirect fluorescent antibody test and different enzyme-linked immunosorbent assays. J. Parasitol. 83:1997;1063-1069
Graham P., Bull B. Approximate standard errors and confidence intervals for indices of positive and negative agreement. J. Clin. Epidemiol. 51:1998;763-771
Hughes H.P.A. The antigenic structure of Toxoplasma gondii. Lyon Med. 248:1982;135-139
Huldt G. Serodiagnosis of parasitic infections. Parasitology. 83:1981;49-55. (Workshop No. 3)
Ihaka R., Gentleman R.R. A language for data analysis and graphics. J. Comput. Graph. Stat. 5:1996;299-314
Jenkins M., Baszler M., Björkman C., Schares G., Williams D. Diagnosis and seroepiemiology of Neospora caninum-associated bovine abortion. Int. J. Parasitol. 32:2002;631-636
Lindsay D.S., Dubey J.P., Duncan R.B. Confirmation that dogs are a definitive host for Neospora caninum. Vet. Parasitol. 82:1999;327-333
McAllister M.M., Dubey J.P., Lindsay D.S., Jolley W.R., Wills R.A., McGuire A.M. Dogs are definitive hosts of Neospora caninum. Int. J. Parasitol. 28:1998;1473-1478
Oswana T., Wastling J., Maley S., Buxton D., Innes E.A.A. Multiple antigen ELISA to detect Neospora-specific antibodies in bovine sera, bovine fœtal fluids, ovine and caprine sera. Vet. Parasitol. 79:1998;19-34
Paré J., Hietala S.K., Thurmond M.C. An enzyme-linked immunosorbent assay (ELISA) for serological diagnosis of Neospora sp. infection in cattle. J. Vet. Diagn. Invest. 7:1995;352-359
Paré J., Fecteau G., Fortin M., Marsolais D. Seroepidemiologic study of Neospora caninum in dairy herd. J. Am. Vet. Med. Assoc. 213:1998;1595-1598
Sawada M., Park C.H., Kondo H., Morita T., Shimada A., Yamane I., Umemura T. Serological survey of antibody to Neospora caninum in Japanese dogs. J. Vet. Med. Sci. 60:1998;853-854
Trees A.J., Guy F., Fennant B.J., Balfour A.H., Dubey J.P. Prevalence of antibodies to Neospora caninum in a population of urban dogs in England. Vet. Rec. 132:1993;125-126
Uggla A., Buxton D. Immune responses against Toxoplasma and Sarcocystis infections in ruminants: diagnosis and prospects for vaccination. Rev. Sci. Tech. Off. Int. Epizoot. 9:1990;441-462
Wouda W., Van den Ingh T.S., Van Knapen F., Sluyter F.J., Koeman J.P., Dubey J.P. Bovine abortion due to Neospora in The Netherlands. Tijdschr. Diergeneesk. 117:1992;509-602
Wouda W., Brinkhof J., Van Maanen C., De Gee A.L.W., Moen A.R. Serodiagnosis of neosporosis in individual cows and dairy herds: a comparative study of three enzyme-linked immunosorbent assays. Clin. Diagn. Lab. Immunol. 5:1995;711-716
Wouda W., Dijkstra T., Kramer A.M.H., Van Maanen C., Brinkhof M.A. Seroepidemiological evidence for a relationship between Neospora caninum infections in dogs and cattle. Int. J. Parasitol. 29:1999;1677-1682
