Dendritic cell; IL-12; Prostaglandin D2; D prostanoid receptor; NF- kB
Abstract :
[en] Prostaglandin (PG) D(2), and its metabolites, are known to be important mediators during acute and chronic inflammation. However, their functions during the early phases of the immune response are poorly documented. In the present study, we show that PGD(2 )inhibits, in a dose-dependent manner, the CD40- and LPS-induced secretion of the Th1-driving factor IL-12 by murine splenic dendritic cells (DC), the most potent antigen-presenting cells. The inhibition of IL-12 production is mediated only in part by the cell surface G alpha s protein-coupled D prostanoid receptor (termed DP1) but not by the G alpha i protein-coupled DP receptor, DP2. We show that recruitment of DP1 in DC results in the activation of a cyclic AMP/protein kinase A pathway that is partially responsible for the inhibition of IL-12 production. We also suggest that the DP1-independent effects exerted by PGD(2) on IL-12 production may be due to the action of ist PGJ(2), but not PGF(2)alpha, metabolites. Electrophoretic mobility shift assays demonstrated that PGD(2) affects NF-kappa B activation through (the) DP1-independent pathway(s). Together these data suggest that PGD(2), by interacting with DP1 and by binding to other target cellular proteins, may regulate immune responses by affecting IL-12 production in DC
Disciplines :
Biochemistry, biophysics & molecular biology Laboratory medicine & medical technology
Author, co-author :
Faveeuw, Christelle; Institut Pasteur de Lille,Inserm U547, Lille, France
Gosset, Philippe; Inserm U416, Institut Pasteur de Lille, Lille, France
Bureau, Fabrice ; Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Angeli, Veronique; Institut Pasteur de Lille, Inserm U547, Lille, France
Hirai, Hiroyuki; R&D Center, BML, Saitama, Japan
Maruyama, Takayuki; Minase Research Institute, Ono Pharmaceutical Co. Ltd., Osaka, Japan
Narumiya, Shuh; Faculty of Medicine, Kyoto University, Kyoto, Japan
Capron, Monique; Institut Pasteur de Lille, Inserm U547, Lille, France
Trottein, Francois; Institut Pasteur de Lille, Inserm U547, Lille, France
Language :
English
Title :
Prostaglandin D2 inhibits the production of interleukin-12 in murine dendritic cells through multiple signaling pathways.
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