Short communication (Scientific journals)
Critical Evaluation of the DNA-methyl Green Assay: Application to Some Indole Alkaloids
Bonjean, Karine; De Pauw-Gillet, Marie-Claire; Bassleer, Roger et al.
1996In Phytotherapy Research, 10, p. 159-S160
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Keywords :
indole alkaloids; methyl green assay; DNA intercalators
Abstract :
[en] A DNA-methyl green colorimetric microassay for the detection of agents that interact with DNA was assessed with reference molecules (doxorubicin, ellipticine, melinonine F, dihydroflavopereirine, cis-Pt, mitomycin C, emetine or colchicine). This test showed some promise as a screening technique for substances that intercalate partly or totally into the DNA helix but it is not suitable for substances that bind covalently to DNA. An application of this test to a number of indole alkaloids was also performed. Cryptolepine, serpentine, alstonine and matadine induced a reduction of the colour of the solution, so indicating an interaction with DNA.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Pharmacy, pharmacology & toxicology
Author, co-author :
Bonjean, Karine ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques
De Pauw-Gillet, Marie-Claire ;  Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Bassleer, Roger ;  Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)
Quetin-Leclercq, Joëlle ;  Université de Liège - ULiège > Département de Pharmacie
Angenot, Luc  ;  Université de Liège - ULiège > Département de Pharmacie
Wright, Colin;  University of Bradford
Language :
English
Title :
Critical Evaluation of the DNA-methyl Green Assay: Application to Some Indole Alkaloids
Publication date :
1996
Journal title :
Phytotherapy Research
ISSN :
0951-418X
eISSN :
1099-1573
Publisher :
Wiley, Chichester, United Kingdom
Volume :
10
Pages :
S-159-S160
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 May 2010

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