Article (Scientific journals)
Purification of active matrix metalloproteinase catalytic domains and its use for screening of specific stromelysin-3 inhibitors.
Kannan, R.; Ruff, M.; Kochins, J. G. et al.
1999In Protein Expression and Purification, 16, p. 76-83
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Keywords :
α-1 proteinase inhibitor; cephalosporin; matrix metalloproteinase inhibitors; stromelysin-3
Abstract :
[en] The matrix metalloproteinase (MMP) stromelysin-3 (ST3) has been shown to be involved in malignant tumor progression and therefore represents an attractive therapeutical target. In order to screen for ST3 synthetic inhibitors, we have produced and purified the catalytic domain of ST3, matrilysin, stromelysin-2, and membrane type-1 MMP from inclusion bodies in a bacterial system. Our strategy allowed the purification of MMPs directly in the active form, thereby avoidingin vitroactivation. A total of 140,000 synthetic compounds from the Bristol-Myers Pharmaceutical Research Institute chemical deck were tested, using a substrate-based colorimetric enzymatic assay, in which ST3 activity was evaluated through its ability to cleave and inactivate α-1 proteinase inhibitor. One ST3 inhibitor belonging to the cephalosporin family of antibiotics was thereby identified.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Kannan, R.
Ruff, M.
Kochins, J. G.
Manly, S. P.
Stoll, I.
El Fahime, E. M.
Noël, Agnès ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Dive, v
Basset, P.
Language :
English
Title :
Purification of active matrix metalloproteinase catalytic domains and its use for screening of specific stromelysin-3 inhibitors.
Publication date :
1999
Journal title :
Protein Expression and Purification
ISSN :
1046-5928
eISSN :
1096-0279
Publisher :
Academic Press, Orlando, United States - Florida
Volume :
16
Pages :
76-83
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 May 2010

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