- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Faculty Research and Publications /
- Microfluidic cell culture systems with integrated sensors...
Open Collections
UBC Faculty Research and Publications
Microfluidic cell culture systems with integrated sensors for drug screening. Grist, Samantha M.; Yu, Linfen; Chrostowski, Lukas; Cheung, Karen C.
Abstract
Cell-based testing is a key step in drug screening for cancer treatments. A microfluidic platform can permit more precise control of the cell culture microenvironment, such as gradients in soluble factors. These small-scale devices also permit tracking of low cell numbers. As a new screening paradigm, a microscale system for integrated cell culture and drug screening promises to provide a simple, scalable tool to apply standardized protocols used in cellular response assays. With the ability to dynamically control the microenvironment, we can create temporally varying drug profiles to mimic physiologically measured profiles. In addition, low levels of oxygen in cancerous tumors have been linked with drug resistance and decreased likelihood of successful treatment and patient survival. Our work also integrates a thin-film oxygen sensor with a microfluidic oxygen gradient generator which will in future allow us to create spatial oxygen gradients and study effects of hypoxia on cell response to drug treatment. In future, this technology promises to improve cell-based validation in the drug discovery process, decreasing the cost and increasing the speed in screening large numbers of compounds. Copyright 2012 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Item Metadata
Title |
Microfluidic cell culture systems with integrated sensors for drug screening.
|
Creator | |
Publisher |
Society of Photo-Optical Instrumentation Engineers (SPIE)
|
Date Issued |
2012
|
Description |
Cell-based testing is a key step in drug screening for cancer treatments. A microfluidic platform
can permit more precise control of the cell culture microenvironment, such as gradients in soluble
factors. These small-scale devices also permit tracking of low cell numbers. As a new screening
paradigm, a microscale system for integrated cell culture and drug screening promises to provide a
simple, scalable tool to apply standardized protocols used in cellular response assays. With the
ability to dynamically control the microenvironment, we can create temporally varying drug profiles
to mimic physiologically measured profiles. In addition, low levels of oxygen in cancerous tumors
have been linked with drug resistance and decreased likelihood of successful treatment and patient
survival. Our work also integrates a thin-film oxygen sensor with a microfluidic oxygen gradient
generator which will in future allow us to create spatial oxygen gradients and study effects of
hypoxia on cell response to drug treatment. In future, this technology promises to improve cell-based
validation in the drug discovery process, decreasing the cost and increasing the speed in screening
large numbers of compounds.
Copyright 2012 Society of Photo-Optical Instrumentation Engineers.
One print or electronic copy may be made for personal use only. Systematic reproduction and distribution,
duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
|
Genre | |
Type | |
Language |
eng
|
Date Available |
2012-03-03
|
Provider |
Vancouver : University of British Columbia Library
|
Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
|
DOI |
10.14288/1.0107565
|
URI | |
Affiliation | |
Citation |
Grist, Samantha M.; Yu, Linfen; Chrostowski, Lukas; Cheung, Karen C. Microfluidic cell culture systems with integrated sensors for drug screening. Microfluidics, BioMEMS, and Medical Microsystems X, edited by Holger Becker, Bonnie L. Gray Proceedings of SPIE, Volume 8251, 825103, 2012.
|
Publisher DOI |
10.1117/12.911427
|
Peer Review Status |
Reviewed
|
Scholarly Level |
Faculty
|
Rights URI | |
Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International