- Library Home /
- Search Collections /
- Open Collections /
- Browse Collections /
- UBC Faculty Research and Publications /
- Melanocyte development in the mouse tail epidermis...
Open Collections
UBC Faculty Research and Publications
Melanocyte development in the mouse tail epidermis requires the Adamts9 metalloproteinase Tharmarajah, Grace T.; Eckhard, Ulrich; Jain, Fagun; Marino, Giada; Prudova, Anna; Urtatiz, Oscar; Fuchs, Helmut; de Angelis, Martin Hrabe; Overall, Christopher M.; Van Raamsdonk, Catherine D.
Abstract
The mouse tail has an important role in the study of melanogenesis, because mouse tail skin can be used to model human skin pigmentation. To better understand the development of melanocytes in the mouse tail, we cloned two dominant ENU-generated mutations of the Adamts9 gene, Und3 and Und4, which cause an unpigmented ring of epidermis in the middle of the tail, but do not alter pigmentation in the rest of the mouse. Adamts9 encodes a widely expressed zinc metalloprotease with thrombospondin type 1 repeats with few known substrates. Melanocytes are lost in the Adamts9 mutant tail epidermis at a relatively late stage of development, around E18.5. Studies of our Adamts9 conditional allele suggest that there is a melanocyte cell autonomous requirement for Adamts9. In addition, we used a proteomics approach, TAILS N-terminomics, to identify new Adamts9 candidate substrates in the extracellular matrix of the skin. The tail phenotype of Adamts9 mutants is strikingly similar to the unpigmented trunk belt in Adamts20 mutants, which suggests a particular requirement for Adamts family activity at certain positions along the anterior-posterior axis.
Item Metadata
| Title |
Melanocyte development in the mouse tail epidermis requires the Adamts9 metalloproteinase
|
| Creator | |
| Contributor | |
| Publisher |
Pigment Cell Melanoma Research
|
| Date Issued |
2018-11
|
| Description |
The mouse tail has an important role in the study of melanogenesis, because mouse tail skin can be
used to model human skin pigmentation. To better understand the development of melanocytes in the mouse tail, we cloned two dominant ENU-generated mutations of the Adamts9 gene, Und3 and Und4, which cause an unpigmented ring of epidermis in the middle of the tail, but do not alter pigmentation in the rest of the mouse. Adamts9 encodes a widely expressed zinc metalloprotease with thrombospondin type 1 repeats with few known substrates. Melanocytes are lost in the Adamts9 mutant tail epidermis at a relatively late stage of development, around E18.5. Studies of our Adamts9 conditional allele suggest that there is a melanocyte cell autonomous requirement for Adamts9. In addition, we used a proteomics approach, TAILS N-terminomics, to identify new Adamts9 candidate substrates in the extracellular matrix of the skin. The tail phenotype of Adamts9 mutants is strikingly similar to the unpigmented trunk belt in Adamts20 mutants, which suggests a particular requirement for Adamts family activity at certain positions along the anterior-posterior axis.
|
| Subject | |
| Genre | |
| Type | |
| Language |
eng
|
| Date Available |
2020-06-17
|
| Provider |
Vancouver : University of British Columbia Library
|
| Rights |
Attribution-NonCommercial-NoDerivatives 4.0 International
|
| DOI |
10.14288/1.0391906
|
| URI | |
| Affiliation | |
| Citation |
Tharmarajah et al. (2018) Melanocyte Development in the Mouse Tail Epidermis Requires the Adamts9 Metalloproteinase. Pigment Cell Melanoma Research 31(6):693-707.]
|
| Publisher DOI |
10.1111/pcmr.12711
|
| Peer Review Status |
Reviewed
|
| Scholarly Level |
Faculty; Researcher
|
| Rights URI | |
| Aggregated Source Repository |
DSpace
|
Item Media
Item Citations and Data
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International