前立腺抽出物(Robaveron)の排尿機能におよぼす影響についての実験的研究3 神経因性膀胱についての生理学的および生化学的実験成績

Abstract

Through the physiological and the biochemical study, the effects of prostatic extract (Robaveron) were investigated on the rabbits with experimental motor neuron lesion by the perfect transsection of the spinal cord. Experiments were carried out with two groups, in which one was transsected the spinal cord at the level of the twelfth dorsal vertebra and another was transsected at the level of the fifth lumbal vertebra after two weeks of operation. The pattern of neurogenic bladder was established within two weeks. From the physiological experiments, it became clear that the sphincter was important as the factor for the lower urinary tract obstruction in the case of upper motor neuron lesion, whereas the bladder neck was more dominant than sphincter in the case of lower motor neuron lesion and that in the lower motor neuron lesion, the acceleration of the vas deferens motility was observed by the stimulation on the urinary bladder, and the regular low voltage contraction in the case of lower motor neuron lesion was the rhythmical contraction of the detrusor muscle itself, moreover it was not influenced by the electrical stimulation of hypogastric nerve. Prostatic extract (Robaveron) increased the elasticity of detrusor muscle and the voiding pressure regardless of upper or lower motor neuron lesion, furthermore in the case of lower motor neuron lesion increased action potential to the rhythmical characteristic contraction of the urinary bladder. On the biochemical study, Robaveron increased permeability of organic acid of TCA cycle member to the mitochondria membrane and result in the elevation of the respiration of TCA cycle. The potent activity of Robaveron was found in the experiment under succinate or oxalacetate, on the comparison of the both, succinate was more efficiently utilized than oxalacetate, while citrate was hardly utilized. Futhermore the respiration of urinary bladder under succinate especially decreased in the case of lower motor neuron lesion, and the decreasing respiration was recovered by injection of Robaveron. From these physiological and biochemical experiments, it was concluded that the beneficial effect of Robaveron on the urinary bladder function was thoroughly myotropic and Robaveron was extensively useful for the improvement in the neurogenic bladder.