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タイトル: | One-step preparation of amino-PEG modified poly(methyl methacrylate) microchips for electrophoretic separation of biomolecules |
著者: | Kitagawa, Fumihiko Kubota, Kei Sueyoshi, Kenji Otsuka, Koji https://orcid.org/0000-0003-1088-0569 (unconfirmed) |
著者名の別形: | 北川, 文彦 |
キーワード: | Microchip electrophoresis Protein analysis Chiral analysis Poly(methyl methacrylate) PEG |
発行日: | 15-Dec-2010 |
出版者: | 2010 Elsevier B.V. |
誌名: | Journal of pharmaceutical and biomedical analysis |
巻: | 53 |
号: | 5 |
開始ページ: | 1272 |
終了ページ: | 1277 |
抄録: | A simple method for a chemical surface modification of poly(methyl methacrylate) (PMMA) microchips with amino-poly(ethyleneglycol) (PEG-NH(2)) by nucleophilic addition-elimination reaction was developed to improve the separation efficiency and analytical reproducibility in a microchip electrophoresis (MCE) analysis of biomolecules such as proteins and enantiomers. In our procedure, the PEG chains were robustly immobilized only by introducing an aqueous solution of PEG-NH(2) into the PMMA microchannel. The electroosmotic mobilities on the modified chips remained almost constant during 35 days with 37 runs without any recoating. The PEG-NH(2) modified chip provided a fast, reproducible, efficient MCE separation of proteins with a wide variety of isoelectric points within 15s. Furthermore, the application of the modified chip to affinity electrophoresis using bovine serum albumin gave a good chiral separation of amino acids. |
著作権等: | © 2010 Elsevier B.V. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/126730 |
DOI(出版社版): | 10.1016/j.jpba.2010.07.008 |
PubMed ID: | 20678876 |
出現コレクション: | 学術雑誌掲載論文等 |
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