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j.antiviral.2008.05.006.pdf | 299.39 kB | Adobe PDF | 見る/開く |
タイトル: | Novel screening systems for HIV-1 fusion mediated by two extra-virion heptad repeats of gp41. |
著者: | Nishikawa, Hiroki Kodama, Eiichi Sakakibara, Ayako Fukudome, Ayako Izumi, Kazuki Oishi, Shinya https://orcid.org/0000-0002-2833-2539 (unconfirmed) Fujii, Nobutaka Matsuoka, Masao |
著者名の別形: | 大石, 真也 児玉, 栄一 |
キーワード: | HIV-1 Gp41 Fusion ELISA Screening Alkaline phosphatase |
発行日: | Oct-2008 |
出版者: | Elsevier BV |
誌名: | Antiviral research |
巻: | 80 |
号: | 1 |
開始ページ: | 71 |
終了ページ: | 76 |
抄録: | Entry of human immunodeficiency virus type 1 (HIV-1) into target cells is mediated by its envelope protein gp41 through membrane fusion. Interaction of two extra-virion heptad repeats (HRs) in the gp41 plays a pivotal role in the fusion, and its inhibitor, enfuvirtide (T-20), blocks HIV-1 entry. To identify agents that block HIV-1 fusion, two screening methods based on detection and quantification by the enzyme-linked immunosorbent assay (ELISA) principle have been established. One method uses an alkaline phosphatase (ALP)-conjugated antibody (Ab-ELISA) and the other uses an ALP-fused HR (F-ELISA) to detect and quantify the interaction of the two HRs. The F-ELISA was more simple and rapid, since no ALP-conjugated antibody reaction was required. Both ELISAs detected all the fusion inhibitors tested except for T-20. Interaction of the two HRs was observed in both ELISAs, even in the presence of 10% dimethyl sulfoxide. Ab-ELISA performed best in a pH ranging from 6 to 8, while F-ELISA performed best at a pH ranging from 7 to 8. These results indicate that both established ELISAs are suitable for the identification of HIV-1 fusion inhibitors. |
著作権等: | © 2008 Elsevier B.V. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/137211 |
DOI(出版社版): | 10.1016/j.antiviral.2008.05.006 |
PubMed ID: | 18584890 |
出現コレクション: | 学術雑誌掲載論文等 |
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