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j.jelechem.2012.11.023.pdf | 1.37 MB | Adobe PDF | 見る/開く |
タイトル: | Electrostatic and steric interaction between redox polymers and some flavoenzymes in mediated bioelectrocatalysis |
著者: | Nieh, Chi-Hua Tsujimura, Seiya Shirai, Osamu Kano, Kenji |
著者名の別形: | 加納, 健司 |
キーワード: | Pentacyanoferrate-bound polymer Osmium complex Flavoenzymes Peroxidase Surface electrostatic potential Electrostatic repulsion |
発行日: | Jan-2013 |
出版者: | Elsevier B.V. |
誌名: | Journal of Electroanalytical Chemistry |
巻: | 689 |
開始ページ: | 26 |
終了ページ: | 30 |
抄録: | H2O2-generating oxidase/peroxidase (POD)-based mediated biosensors are very useful to minimize interference, but require suitable mediators which work well only for POD but not against the oxidase. Pentacyanoferrate-bound poly(1-vinylimidazole) (PVI[Fe(CN)5]), PVI[Os(dcbbpy)2Cl] (dcbbpy = 4, 4′-dicarboxy-2, 2′-bipyridine) and PVI[Os(dmebpy)2Cl] (dmebpy = 4, 4′-dimethyl-2, 2′-bipyridine) have been utilized to investigate the interaction with four kinds of H2O2-generating oxidases: glucose oxidase, sarcosine oxidase, choline oxidase (ChOD) and lactate oxidase. The mediated bioelectrocatalytic activities of the redox polymers for the enzymes have been determined by cyclic voltammetry in the presence of the substrates. The highly negatively charged PVI[Fe(CN)5] shows practically no mediating activity against the four flavoenzymes, but strong one to POD. On the other hand, PVI[Os(dmebpy)2Cl] with neutral ligands shows a high activity for the oxidases except ChOD. The mediating activity of PVI[Os(dcbbpy)2Cl] with negatively charged ligands is much smaller than that of PVI[Os(dmebpy)2Cl]. These results reveal that electrostatic repulsion and steric hindrance are enhanced by using negatively charged polymers to realize minimum activity against the oxidases. |
著作権等: | © 2012 Elsevier B.V. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/168710 |
DOI(出版社版): | 10.1016/j.jelechem.2012.11.023 |
出現コレクション: | 学術雑誌掲載論文等 |
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