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タイトル: | Dialysis Purification of Integrase-DNA Complexes Provides High-Resolution Atomic Force Microscopy Images: Dimeric Recombinant HIV-1 Integrase Binding and Specific Looping on DNA. |
著者: | Tsuruyama, Tatsuaki Nakai, Tonau Ohmori, Rei Ozeki, Munetaka Tamaki, Keiji Yoshikawa, Kenichi |
著者名の別形: | 鶴山, 竜昭 |
発行日: | 14-Jan-2013 |
出版者: | Public Library of Science |
誌名: | PloS one |
巻: | 8 |
号: | 1 |
論文番号: | e53572 |
抄録: | It remains difficult to obtain high-resolution atomic force microscopy images of HIV-1 integrase bound to DNA in a dimeric or tetrameric fashion. We therefore constructed specific target DNAs to assess HIV-1 integrase binding and purified the complex by dialysis prior to analysis. Our resulting atomic force microscopy analyses indicated precise size of binding human immunodeficiency virus type 1 (HIV-1) recombinant integrase in a tetrameric manner, inducing formation of a loop-like or figure-eight-like secondary structure in the target DNA. Our findings regarding the target DNA secondary structure provide new insights into the intermediate states of retroviral integration. |
著作権等: | © 2013 Tsuruyama et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/169671 |
DOI(出版社版): | 10.1371/journal.pone.0053572 |
PubMed ID: | 23341952 |
出現コレクション: | 学術雑誌掲載論文等 |
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