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タイトル: | Structural fluctuation observed in Z-DNA d(CGCGCG)2 in the absence of divalent metal cations and polyamines. |
著者: | Chatake, Toshiyuki https://orcid.org/0000-0001-9208-9951 (unconfirmed) |
著者名の別形: | 茶竹, 俊行 |
キーワード: | Z-DNA Na^+ ion ZI-ZII conformation hydration structural fluctuation |
発行日: | Nov-2013 |
出版者: | International Union of Crystallography |
誌名: | Journal of synchrotron radiation |
巻: | 20 |
号: | Part 6 |
開始ページ: | 864 |
終了ページ: | 868 |
抄録: | In the present study, Z-DNA d(CGCGCG)2 was crystallized from a DNA solution in the absence of divalent metal cations and polyamines, and its X-ray structure was determined at 0.98 Å resolution. Comparison of this structure and previously reported Z-DNA structures, containing Mg(2+) cations and/or polyamines, demonstrated that Z-DNA can have structural fluctuations with respect to phosphate groups and hydration in the minor groove. At the GpC steps, a two-state structural equilibrium between the ZI and ZII conformations was frequently observed. In contrast, at the CpG steps, the phosphate groups exhibited rotational fluctuation, which could induce distortion of sugar puckering. In addition, alternative positions of water molecules were found in the middle of the minor groove of the Z-DNA. These structural fluctuations were likely observable because of the absence of Mg(2+) cations and polyamines. The results related to these phenomena were supported by those of other experimental methods, suggesting the possibility of these fluctuations occurring in biological conditions. |
著作権等: | © International Union of Crystallography |
URI: | http://hdl.handle.net/2433/179414 |
DOI(出版社版): | 10.1107/S0909049513020773 |
PubMed ID: | 24121329 |
出現コレクション: | 学術雑誌掲載論文等 |
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