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ファイル | 記述 | サイズ | フォーマット | |
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j.chembiol.2014.07.019.pdf | 1.2 MB | Adobe PDF | 見る/開く |
タイトル: | Targeted Suppression of EVI1 Oncogene Expression by Sequence-Specific Pyrrole-Imidazole Polyamide. |
著者: | Syed, Junetha Pandian, Ganesh N Sato, Shinsuke Taniguchi, Junichi Chandran, Anandhakumar Hashiya, Kaori Bando, Toshikazu Sugiyama, Hiroshi https://orcid.org/0000-0001-8923-5946 (unconfirmed) |
著者名の別形: | 杉山, 弘 |
発行日: | 23-Oct-2014 |
出版者: | Elsevier Ltd. |
誌名: | Chemistry & biology |
巻: | 21 |
号: | 10 |
開始ページ: | 1370 |
終了ページ: | 1380 |
抄録: | Human ectopic viral integration site 1 (EVI1) is an oncogenic transcription factor known to play a critical role in many aggressive forms of cancer. Its selective modulation is thought to alter the cancer-specific gene regulatory networks. Pyrrole-imidazole polyamides (PIPs) are a class of small DNA binders that can be designed to target any destined DNA sequence. Herein, we report a sequence-specific pyrrole-imidazole polyamide, PIP1, which can target specific base pairs of the REL/ELK1 binding site in the EVI1 minimal promoter. The designed PIP1 significantly inhibited EVI1 in MDA-MB-231 cells. Whole-transcriptome analysis confirmed that PIP1 affected a fraction of EVI1-mediated gene regulation. In vitro assays suggested that this polyamide can also effectively inhibit breast cancer cell migration. Taken together, these results suggest that EVI1-targeted PIP1 is an effective transcriptional regulator in cancer cells. |
著作権等: | © 2014 Elsevier Ltd. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/191246 |
DOI(出版社版): | 10.1016/j.chembiol.2014.07.019 |
PubMed ID: | 25219965 |
出現コレクション: | 学術雑誌掲載論文等 |
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