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タイトル: | Effect of a Low-conductivity Zone on Field-amplified Sample Stacking in Microchip Micellar Electrokinetic Chromatography |
著者: | Sueyoshi, Kenji Kitagawa, Fumihiko Otsuka, Koji https://orcid.org/0000-0003-1088-0569 (unconfirmed) |
発行日: | 2013 |
出版者: | Japan Society for Analytical Chemistry |
誌名: | Analytical Sciences |
巻: | 29 |
号: | 1 |
開始ページ: | 133 |
終了ページ: | 138 |
抄録: | The effects of a low-conductivity (low-C) zone of an on-line sample preconcentration based on field-amplified sample stacking (FASS) under an acidic condition was investigated in microchip micellar electrokinetic chromatography (MCMEKC). By employing originally fabricated microchips with T- and cross-channel injectors on a single device, partial injection of the low-C zone and large-volume injection of a sample solution were demonstrated. As a typical result, the observed peak intensities of the fluorescent dyes were 1.5 – 2.0-fold enhanced by partial injection of the low-conductivity zone, as compared to those in FASS-MCMEKC without injecting the low-C zone; also, 30 – 40-fold enhancements of the detectability were achieved as compared to the conventional MCMEKC analyses. The resolutions of the hydrophobic analytes were also improved by introducing the low-C zone. The difference in the retention factors of model analytes in the high-C background and low-C zones was also estimated in order to clarify the effect of the low-C zone on the resolution and detectability in FASS-MCMEKC. |
著作権等: | © The Japan Society for Analytical Chemistry 2013 |
URI: | http://hdl.handle.net/2433/193726 |
DOI(出版社版): | 10.2116/analsci.29.133 |
PubMed ID: | 23303098 |
出現コレクション: | 学術雑誌掲載論文等 |
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